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不同形态氮肥降低菜心吸收累积环丙沙星(CIP) 的生理生化机制
杜明慧,成玉婷,吴小莲,霍锦林,何子豪,陈永骥,何坤航,洪文泓,王海龙
0
(佛山科学技术学院食品科学与工程学院,佛山 528000;湖南郴电国际发展股份有限公司,郴州 432000;佛山科学技术学院环境与化学工程学院,佛山 528000)
摘要:
以抗生素环丙沙星(Ciprofloxacin,CIP)为目标化合物,以我国南方地区重要蔬菜作物菜心(Brassica parachinensis L.)为供试作物,采用盆栽实验研究4种形态氮肥(酰胺态氮、硝态氮、铵态氮和氰氨态氮)处理下菜心组织中CIP含量及菜心生理生化特征,并从菜心生理生化角度探讨了不同形态氮肥对菜心CIP吸收和转运能力的差异机制。结果表明,4种氮肥均显著降低了菜心组织中CIP含量,且不同形态氮肥对菜心CIP富集产生显著差异,其菜心地上部和根系中CIP含量依次为酰胺态氮<铵态氮<硝态氮<氰氨态氮,其含量与对照组相比降低了20.7% ~ 62.0%,且不同形态氮肥均降低了菜心根部富集系数(BCF)和茎叶转运系数(TF),其中酰胺态氮和铵态氮肥对BCF和TF抑制作用最为显著。同时,施用氮肥均显著提高了菜心生物量、叶片硝酸还原酶活性、叶绿素(a和b)的含量,结合分析荧光动力曲线和叶片光系统II(PS II)叶绿素荧光参数,发现氮肥能有效缓解CIP对菜心叶片PSⅡ中电子传递链的干扰和破坏,且能促进光合作用电子传递过程进而提高叶片光合作用性能,特别是酰胺态氮肥的促进作用最为显著。综上所述,可通过施用不同形态氮肥来调控作物生长和作物体内污染物富集水平,结果可为农田作物安全生产提供科学依据。
关键词:  菜心  环丙沙星  氮肥  抗生素  生理生化
DOI:10.13610/j.cnki.1672-352x.20220907.018
基金项目:国家自然科学基金(4907344)资助。
Mechanism of different nitrogen fertilizer forms on transportation of Ciprofloxacin by Chinese flowering cabbage
DU Minghui,CHENG Yuting,WU Xiaolian,HUO Jinlin,HE Zihao,CHEN Yongji,HE Kunhang,HONG Wenhong,WANG Hailong
(School of Food Science and Engineering, Foshan University, Foshan 528000;Hunan Chendian International Development Co.,Ltd., Chenzhou 423000;School of Environmental and Chemical Engineering, Foshan University, Foshan 528000)
Abstract:
A pot experiment was performed to study the Ciprofloxacin (CIP) content in the tissue of Chinese cabbage (Brassica parachinensis L.) and the characteristics of physiological and biochemical treated with four different nitrogen forms (amide nitrogen, nitrate nitrogen, ammonium nitrogen and cyanamine nitrogen). The influence mechanism of different forms of nitrogen fertilizer on absorption and transport capacity of CIP in Chinese cabbage was discussed based on the perspective of physiology and biochemistry. The results showed that all nitrogen fertilizers significantly reduced the CIP content in Chinese cabbage tissue, and significant differences were in the CIP enrichment among four nitrogen forms. The CIP content in the shoot and root of Chinese cabbage was in the order of amide nitrogen < ammonium nitrogen < nitrate nitrogen < cyanamide nitrogen, which decreased by 20.7% - 62.0% compared with the control group. The root enrichment coefficient (BCF) and the stem leaf transport coefficient (TF) of Chinese cabbage were reduced by nitrogen fertilizer, and among which amide nitrogen and ammonium nitrogen had the most significant inhibitory effects on BCF and TF. At the same time, the application of nitrogen fertilizer significantly increased the biomass, nitrate reductase activity and chlorophyll (a and b) content of Chinese cabbage leaves. Combined with the analysis of fluorescence dynamic curve and chlorophyll fluorescence parameters of leaf
Key words:  Chinese flowering cabbage  ciprofloxacin  nitrogen fertilizer  antibiotics  physiology and biochemistry characteristics

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