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桑叶叶际和家蚕肠道细菌群落结构及功能分析
刘桂伽,陈永静,孙庆业,张明珠
0
(安徽大学资源与环境工程学院,安徽省矿山生态修复工程实验室,合肥 230601)
摘要:
家蚕是我国重要的经济昆虫,主要以桑叶为食,了解家蚕肠道及所食桑叶叶际微生物对减少家蚕病害具有重要意义。以5龄第3天的家蚕幼虫和所食桑叶为研究对象,利用高通量测序技术对家蚕肠道和叶际细菌的16S rDNA V3-V4区序列进行测序分析。结果表明:桑叶叶际和家蚕肠道各部位细菌的多样性指数(Shannon指数和Simpson指数)无显著差异(P > 0.05);NMDS分析显示桑叶叶际细菌与家蚕肠道细菌各自聚类,家蚕肠道各部分的细菌菌群无显著差异。桑叶叶际和家蚕肠道中共注释到的主要分类阶元有29 个门、268 个属:前4个优势门为变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)和放线菌门(Actinobacteria);芽孢杆菌属Bacillus为桑叶叶际细菌的最优势属,泛菌属Pantoea为家蚕前肠细菌的最优势属,鞘氨醇单胞菌属Sphingomonas为家蚕中肠和后肠细菌的最优势属。通过功能预测可知,消化系统、免疫系统、能量代谢、代谢性疾病和传染性疾病在家蚕的前肠、中肠中占比较高,氨基酸代谢、碳水化合物代谢、神经退行性疾病、免疫系统疾病、脂类代谢、其他氨基酸代谢、心血管疾病和核苷酸代谢在家蚕的后肠中占比较高。家蚕肠道细菌群落与叶际细菌群落前4个优势菌门一致;家蚕肠道的前肠和中肠功能菌群相似,与后肠差异较大。
关键词:  家蚕  叶际微生物  肠道细菌  群落结构  功能预测
DOI:10.13610/j.cnki.1672-352x.20220907.012
投稿时间:2021-12-24
基金项目:安徽高校协同创新项目(GXXT-2021-061)资助。
Analysis of bacterial community structure and function of phyllosphere in mulberry leaf and Bombyx mori intestine
LIU Guijia,CHEN Yongjing,SUN Qingye,ZHANG Mingzhu
(School of Resources and Environmental Engineering, Anhui Province Engineering Laboratory for Mine Ecological Remediation, Anhui University, Hefei 230601)
Abstract:
The silkworm (Bombyx mori), a specialist herbivore that feeds on mulberry leaves, is an economically important insect in China. Exploring the bacteria of silkworm intestine and phyllosphere in mulberry leaf is of great significance to reduce silkworm diseases. Taken silkworm larvae on the third day of the fifth instar and mulberry leaves feeding as the research object, the 16S rDNA gene fragments (V3-V4 region) of bacteria in silkworm intestine and phyllosphere of mulberry leaf were sequenced by Illumina MiSeq. The results demonstrated that there was no significant difference (P > 0.05) among the diversity indexes (Shannon index and Simpson index) of bacteria in phyllosphere of mulberry leaf and each particular location in silkworm intestine. NMDS analysis showed that the bacteria in phyllosphere of mulberry leaf and silkworm intestine clustered separately, and there was no significant difference between the bacteria in various parts of the silkworm intestine. Analysis results indicated that the bacteria in the phyllosphere of mulberry leaf and the intestine of silkwormwere annotated into 29 phyla and 268 genera, and four phyla (Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria) were predominant populations. Bacillus, Pantoea and Sphingomonas were the predominant genera in the phyllosphere of mulberry leaf, the foregut of silkworm, and midgut and hindgut of silkworm, respectively. Functional prediction analysis showed that digestive system, immune system, energy metabolism, metabolic diseases and infectious diseases accounted for a higher proportion in the silkworm foregut and midgut, but amino acid metabolism, carbohydrate metabolism, neurodegenerative diseases, immune system diseases, lipid metabolism, metabolism of other
Key words:  Bombyx mori  phyllosphere microbe  intestinal bacteria  community structure  function prediction

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