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基于SNP分子标记的宁夏4个野生枣种群遗传多样性对比分析
王博,严亮亮,张悦,宋丽华
0
(宁夏大学农学院,银川 750021)
摘要:
在实地勘察的基础上,以宁夏不同生态立地分布的野生酸枣为材料,对宁夏境内4个野生酸枣种群的遗传多样性进行分析,为宁夏野生酸枣资源可持续原位保存及创新利用提供理论依据。在宁夏选取4个地域代表性的不同野生酸枣种群,从中分别随机选取植株30棵树,采集其叶片,采用新型的基因组及分子标记SNP(单核苷酸多态性)技术,从分子水平上揭示宁夏4个野生酸枣的遗传结构和进化关系。主要研究结果如下:通过SNP标记评价宁夏野生酸枣种群的遗传特性,地理分布上可分为两个主要地理类群,分别代表宁夏中部和北部地区,中部地区包括吴忠红寺堡种群,北部地区包括中卫日照山、灵武白芨滩与贺兰山滚钟口种群;野生酸枣种群之间的亲缘关系表现为贺兰山滚钟口种群与灵武白芨滩种群更接近,而中卫日照山种群和吴忠红寺堡种群各自单独成一类。中卫日照山种群与灵武白芨滩种群遗传分化程度最高,其Fst值为0.191,分化程度较大,分化程度最低的是宁夏贺兰山滚钟口种群与灵武白芨滩种群,其Fst为0.084,属中等分化程度。
关键词:  野生酸枣  SNP标记  遗传多样性
DOI:10.13610/j.cnki.1672-352x.20220705.016
投稿时间:2021-06-06
基金项目:宁夏自然科学基金(2021AAC03110)和宁夏重点研发项目(2018BFH03015)共同资助。
Genetic diversity analysis of four wild Jujube populations in Ningxia based on SNP molecular markers
WANG Bo,YAN Liangliang,ZHANG Yue,SONG Lihua
(School of Agriculture, Ningxia University, Yinchuan 750021)
Abstract:
In order to provide a theoretical basis for the sustainable in-situ conservation and innovative utilization of wild jujube resources in Ningxia, four representative wild jujube populations in different ecological sites in Ningxia were selected to analyze the genetic diversity in Ningxia on the basis of the field survey, in which 30 trees were randomly selected from the four wild jujube populations, and the leaves were collected. A novel genomic and molecular marker SNP(single nucleotide polymorphism)technique was used, and the genetic structure and evolutionary relationship of the four wild jujubes from Ningxia were revealed on the molecular level. The main research results were as follows. The genetic characteristics of wild jujube populations in Ningxia were evaluated by SNP markers, and the geographical distribution could be divided into two main geographical groups, which represented the central and northern regions of Ningxia, respectively. The central region included Wuzhong Hongsibao population, and the northern region included Zhongwei Rizhaoshan population, Lingwu Baijitan population and Helanshan Gunzhongkou population. The genetic relationship between the wild jujubes populations was that Helanshan Gunzhongkou population was more similar to Lingwu Baijitan population, while Zhongwei Rizhaoshan population and Wuzhong Hongsipu population were separated into a single group. The genetic differentiation degrees of Zhongwei Rizhaoshan population and Lingwu Baijitan population were the highest, with the Fst of 0.191; the lowest degree of differentiation was Helanshan Gunzhongkou population and Lingwu Baijitan population, with the Fst of 0.084, belonging to medium differentiation.
Key words:  Ziziphus jujuba Mill. var. spinosa  SNP marker  genetic diversity

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