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基于SSR标记研究香谷繁殖更新群体量和发芽率临界值
李霞,黎毛毛,刘进,周慧颖,胡佳晓,王晓玲,余丽琴
0
(江西省农业科学院水稻研究所/江西省农作物种质资源研究中心,南昌 330200)
摘要:
对地方水稻品种繁殖更新群体量和发芽率临界值进行研究,有利于为种质库中地方水稻品种繁殖更新适宜群体量和发芽率临界值的制定提供科学依据。利用地方水稻品种香谷为试验材料,通过人工老化处理获得不同发芽梯度的香谷群体,采用农艺性状观察和SSR分子标记分析繁殖更新群体大小与发芽率水平对其遗传完整性的影响。农艺性状观察表明保持香谷遗传结构完整的繁殖更新群体量为60~140株,利用SSR分子标记进一步分析表明,繁殖群体扩大到200株时,更利于保证种质遗传完整性。同时,SSR分析表明发芽率低于60%(50%与≤30%)群体的等位基因数、有效等位基因数、基因多样性指数、香农指数均与对照呈极显著差异;发芽率为60%~70%的群体除香农指数与对照达到了显著差异外,等位基因数、有效等位基因数、基因多样性指数与对照差异不显著。地方水稻品种香谷适宜的繁殖更新群体量为200 株,繁殖更新的发芽率临界值为60%~70%。
关键词:  水稻  香谷  繁殖更新群体量  发芽率临界值  遗传完整性
DOI:10.13610/j.cnki.1672-352x.20220705.012
投稿时间:2021-09-06
基金项目:国家作物种质资源库“野生稻江西分库”(NCGRC-2020-050), 江西省农业科学院博士启动项目(20181CBS0030)和水稻生物学国家重点实验室开放课题(20200201)共同资助。
Study on regeneration population size and the germination rate threshold of Xianggu by SSR markers
LI Xia,LI Maomao,LIU Jin,ZHOU Huiying,HU Jiaxiao,WANG Xiaoling,YU Liqin
(Rice Research Institute, Jiangxi Academy of Agricultural Sciences /Crop Germplasm Resources Research Center of Jiangxi Province, Nanchang 330200)
Abstract:
The study on the regeneration population size and the germination rate threshold of rice landraces will provide a scientific basis for the establishment of the size of reproduction population and the critical value of germination rate during the reproduction and renewal of rice landraces germplasm resources in the seed bank. Xianggu, a rice landrace, was used as experiment material, and the effects of regeneration population size and germination rate on the genetic integrity of rice landrace were studied by combined investigation of agronomic traits and SSR molecular marker analysis. The investigation of agronomic traits showed that: to maintain the integrity of Xianggu genetic structure, the appropriate regeneration population size was 60 - 140. Further verification by SSR molecular markers showed that when the regeneration population expanded to 200 plants, it was benefit to guarantee the genetic integrity of germplasm. Meanwhile, the number of alleles, number of effective alleles, gene diversity and Shannon index of populations with germination rate below 60% (50% and ≤ 30%) were extremely significantly lower than those of the control. Except the Shannon index, the number of alleles, number of effective alleles and gene diversity index of populations with germination rate 60% - 70% were not significantly different from those of the control. The Shannon index of the population with 60% - 70% germination rate was significantly lower than that of the control. The result indicated that the optimal regeneration population size of Xianggu was 200 and the critical value of germination rate was 60% -70% during reproductive renewal of Xianggu.
Key words:  rice  Xianggu  regeneration population  critical value of germination rate  genetic integrity

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