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黄山松成熟胚培养与植株再生技术研究
兰延钢,汤明霞,许成芳,王瑞佳,严涵薇,吴敏,项艳
0
(安徽农业大学林学与园林学院,合肥 230036;黄山风景区管理委员会园林局,黄山 245899)
摘要:
以黄山松(Pinus taiwanensis Hayata)成熟胚为试验材料,进行离体培养与植株再生条件的初步研究。结果表明,黄山松成熟胚外植体表面灭菌以75%的乙醇处理1 min,3%的次氯酸钠处理10 min为适宜;愈伤组织诱导培养基以MS + 1.0 mg·L-1 6-BA + 0.2 mg·L-1 NAA为最佳;适宜的不定芽分化培养基为DCR + 1.0 mg·L-1 6-BA + 0.05 mg·L-1 NAA;不定芽伸长以DCR + 0.1 mg·L-1 6-BA + 0.05 mg·L-1 NAA为适宜;生根培养基以1/2 DCR + 2.0 mg·L-1 IBA + 0.05 mg·L-1 NAA为适宜。建立的植株再生体系为黄山松种质资源的保存、遗传改良及优质种苗繁殖等研究提供了参考。
关键词:  黄山松  成熟胚  离体培养  植株再生
DOI:10.13610/j.cnki.1672-352x.20220705.006
基金项目:全球环境基金(GEF)赠款、联合国粮农组织(FAO)为国际执行机构、黄山风景区代表黄山市人民政府实施的“黄山地区生物多样性保护和可持续利用”项目资助。
Research on mature embryo culture and plant regeneration of Pinus taiwanensis Hayata
LAN Yangang,TANG Mingxia,XU Chengfang,WANG Ruijia,YAN Hanwei,WU Min,XIANG Yan
(School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036;Landscape Bureau of Huangshan Scenic Area Management Committee, Huangshan 245899)
Abstract:
In this study, the mature embryo of Pinus taiwanensis Hayata was used as the experimental material to conduct a preliminary study of in vitro culture and plant regeneration conditions. The results showed that the suitable surface sterilization method for mature embryos of P. taiwanensis Hayata was 75% alcohol treatment for 1 min and 3% sodium hypochlorite for 10 min; the optimal callus induction medium was MS + 1.0 mg·L-1 6-BA + 0.2 mg·L-1 NAA; the optimal induction medium for adventitious buds was DCR + 1.0 mg·L-1 6-BA + 0.05 mg·L-1 NAA; the suitable induction condition for adventitious bud elongation was DCR + 0.1 mg·L-1 6-BA + 0.05 mg·L-1 NAA; the optimal medium for rooting was 1/2 DCR + 2.0 mg·L-1 IBA + 0.05 mg·L-1 NAA. The results obtained in this study will be of great significance for the preservation, genetic improvement and high-quality seedling reproduction of P. taiwanensis Hayata germplasms.
Key words:  Pinus taiwanensis Hayata  mature embryo  in vitro culture  plant regeneration

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