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基于网络药理学黄精抗炎活性成分及作用机制研究
许慧,代磊,邓鹏飞,徐小牛
0
(安徽农业大学林学与园林学院,合肥 230036)
摘要:
黄精属百合科黄精属,性味甘甜,食用爽口,具有重要的药用和食用价值。为探讨黄精抗炎可能活性成分及作用机制,借助TCMSP数据库筛选黄精活性成分,利用Pharmmapper和Uniprot数据库查找黄精活性成分靶点基因,借助GeneCards和MalaCards数据库筛选人体炎症相关基因,取交集获取黄精抗炎潜在靶点;在Metascape数据库进行GO富集和KEGG通路注释;使用Cytoscape3.6.1软件构建黄精抗炎潜在靶点的“成分-靶点-通路”网络图,STRING数据库构建PPI网络图。共筛选出(+)-丁香树脂醇-O-β-D-葡萄糖苷、中华在线1、甲基原纤细薯蓣皂苷、西伯利亚蓼苷A、3’-甲氧基大豆苷元、β-谷甾醇等12个潜在活性成分和ALB、EGFR、MAPK1、CASP3、ESR1等65个黄精抗炎潜在靶标,生物信息学富集分析中筛选出46个GO条目和13条KEGG通路,主要涉及Pathways in cancer(癌症的途径)、Prostate cancer(前列腺癌)、IL-17 signaling pathway(IL-17信号通路)、Hepatitis B(乙型肝炎)通路。该研究揭示了黄精抗炎多成分、多靶点、多通路的作用机制,为黄精抗炎机制研究和后续试验研究提供依据。
关键词:  黄精  抗炎  网络药理学  分子机制
DOI:10.13610/j.cnki.1672-352x.20220325.020
投稿时间:2021-04-06
基金项目:安徽省科技计划(20937001), 国家重点研发计划(2018YFD0600105)和安徽农业大学研究生创新基金(2021yis-13)共同资助。
Study on the anti-inflammatory active components and mechanism of Polygonati-rhizoma based on network pharmacology
XU Hui,DAI Lei,DENG Pengfei,XU Xiaoniu
(School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036)
Abstract:
Polygonati rhizoma has important medicinal and edible values, tastes sweet and refreshing. To explore the possible anti-inflammatory active components and mechanism of Polygonati rhizoma, the active components in Polygonati rhizoma were screened by TCMSP database; the target genes related to Polygonati rhizoma active components were found in Pharmmapper and UniProt database, human inflammatory related genes were screened using GeneCards and Malacards databases, and the potential anti-inflammatory targets of Polygonati rhizoma were obtained by intersection; GO enrichment and KEGG pathway annotation were carried out by Metascape database; the "Compound-Target-Disease" network diagram of potential anti-inflammatory targets of Polygonati rhizoma was constructed by using Cytoscape3.6.1 software, and PPI network diagram was constructed by using string database. A total of 12 potential active components, including (+)-Syringaresinol-O-beta-D- glucoside, zhonghualiaoine 1, Diosgenin, 3'-Methoxydaidzein, Beta-Sitosterol, Methylprotodioscin and 65 potential anti-inflammatory targets of Polygonati rhizoma, such as ALB, EGFR, MAPK1, CASP3, ESR1, etc., were screened out, and 46 GO items and 13 KEGG items were screened out in bioinformatics enrichment analysis. The pathways mainly involve in cancer, prostate cancer, IL-17 signaling pathway and hepatitis B. The study reveals the mechanism of anti-inflammatory multi-component, multi-target and multi-channel of Polygonati rhizoma, which provides a basis for the research of anti-inflammatory mechanism of Polygonati rhizoma and the subsequent experimental research.
Key words:  Polygonati rhizoma  anti-inflammatory  network pharmacology  molecular mechanism

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