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复合酶法提取蓝莓皮渣中白藜芦醇的工艺及其分离提纯
张华,仇宏图,高青山,王福超
0
(延边大学农学院,延吉 133002;延边大学农学院,延吉 133002; 锡林郭勒职业学院,锡林浩特 026000)
摘要:
为提高蓝莓皮渣这一副产品的利用率,通过冷冻干燥技术,制得蓝莓皮渣粉末,通过单因素试验确定复合酶比例、酶解温度、酶解时间、pH值4个因素对蓝莓皮渣中白藜芦醇的含量的影响,采用响应面优化得出最优提取条件,并进行了LC-18SPE分离提纯。结果表明:液相色谱-质谱法(LC-MS)结果显示蓝莓皮渣中大多为酯类物质和醛、酮类物质;白藜芦醇最优提取条件为料液(乙醇)比1:20(g·mL-1),复合酶比例(纤维素酶:果胶酶)1:5,酶解温度54.62 ℃,酶解时间1 h,pH值5.38,白藜芦醇提取含量最高为(236 ±0.424) mg·kg-1。利用乙酸乙酯分提含量最高达到76.61%。
关键词:  蓝莓皮渣  复合酶法  白藜芦醇  分离提纯
DOI:10.13610/j.cnki.1672-352x.20211026.001
基金项目:高等学校学科创新引智计划111项目(D20034)资助。
Extraction resveratrol process and fractionation from blueberry peel residue using mulriple enzymes
ZHANG Hua,QIU Hongtu,GAO Qingshan,WANG Fuchao
(School of Agronomy, Yanbian University, Yanji 133002;School of Agronomy, Yanbian University, Yanji 133002; Xilingol Vocational College, Xilinhaote 026000)
Abstract:
Blueberry peel power (BPP) was obtained by freeze-drying technology to improve the utilization rate of blueberry peel as a by-product. The contents of resveratrol from blueberry powder were determined by single experiments on the ratio of complex enzymes, enzymolysis temperature, enzymolysis time and pH values to explore the optimal conditions, and LC-18SPE was used to separate and purify BPP, which was mainly composed of esters, aldehydes and ketones. The optimal conditions for BPP extraction were based on the ratio of solid (BBP) to liquid (ethanol) of 1:20 (g·mL-1), the ratio of compound enzyme (cellulase: pectinase) of 1:5, enzymolysis at 54.62 ℃ for 1 h with pH value of 5.38, and the extraction rate of resveratrol content was (236±0.424) mg·kg-1. The fractionation content with ethyl acetate was up to 76.61%.
Key words:  blueberry pericarp  combined-enzyme method  resveratrol  fractionation

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