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希瓦氏菌对甲基绿的脱色及其机制
鲍巧雨,钱怀丽,郭晶晶,邓国志,武超
0
(安徽大学资源与环境工程学院,合肥 230601; 湿地生态保护与修复安徽省重点实验室(安徽大学),合肥 230601)
摘要:
以希瓦氏菌(Shewanella oneidensis MR-1)为染料脱色菌株对甲基绿(methyl green,MG)进行脱色处理,研究不同碳源、甲基绿浓度、初始菌株浓度和温度对染料脱色的影响。通过紫外-可见吸收光谱(UV-visible)、傅里叶红外光谱(FTIR)以及液相色谱-质谱联用(LC-MS)对甲基绿的脱色产物进行分析研究,通过杀菌实验去测定甲基绿脱色液的生物毒性,通过S.oneidensis MR-1及其突变菌株对甲基绿的脱色速率推测可能的生物机制。研究结果表明:S. oneidensis MR-1在以甲酸钠为碳源时的脱色率最高;在不同甲基绿浓度下,该菌对40 mg·L-1甲基绿可在1 h内脱色率达95%以上;菌株浓度的增加会增强脱色效果;温度研究显示,S. oneidensis MR-1在30 ℃条件时,对甲基绿的脱色效果更好。产物分析及杀菌实验结果证实S. oneidensis MR-1能够对甲基绿进行脱色,脱色后液体无毒,脱色产物主要为:4-(N,N-二甲基氨基)-4′-(N′,N′-二甲基氨基)二苯甲酮(DDBP),4-(N-乙基-N,N-二甲基氨基)-4′-(N′-甲基氨基)二苯甲酮(ED-MBP), 4-(N-乙基-N,N-甲基氨基)-4′-(N′,N′-二甲基氨基)二苯甲酮(EM-DBP)和4-(N-乙基-N,N-甲基氨基)-4′-(N′-甲基氨基)二苯甲酮(ED-BP)。
关键词:  希瓦氏菌  甲基绿  脱色  产物分析  毒性  生物机制
DOI:10.13610/j.cnki.1672-352x.20220106.013
基金项目:国家自然科学基金(31670126)和安徽省住房城乡建设科学技术计划项目(2017YF-03)共同资助。
Decolorization of methyl green by Shewanella oneidensis MR-1 and its mechanism
BAO Qiaoyu,QIAN Huaili,GUO Jingjing,DENG Guozhi,WU Chao
(School of Resources and Environmental Engineering, Anhui University, Hefei 230601; Anhui Province Key Laboratory of Wetland Ecosystem Protection and Restoration (Anhui University), Hefei 230601)
Abstract:
The present study was conducted using Shewanella oneidensis MR-1 for the decolorization of triphenylmethane dye methyl green to evaluate the effects of various parameters (different carbon sources, initial concentrations of methyl green and S. oneidensis MR-1and temperature) on methyl green removal. Decolorizing products of methyl green were analyzed by UV-visible,FTIR analysis and LC-MS analyses. The toxicity of methyl green decolorizing solution was determined by the killing assay. The possible biological mechanism was inferred from the decolorization rate of methyl green by S. oneidensis MR-1 wild-type and mutant strains. The results showed that S. oneidensis MR-1 had the highest decolorization rate when the carbon source was formic acid. Under different concentrations of methyl green, the decolorization efficiency was up to 95% after 1 h when methyl green concentration was 40 mg·L-1. The decolorization efficiency was enhanced with the increase of strain concentration. Temperature experiment showed that the decolorization efficiency of methyl green was better at 30 ℃. The results of product analysis and killing assay confirmed that S. oneidensis MR-1 could decolorize methyl green and the decolorizing solution had no obvious toxicity. The decolorizing products of methyl green included 4-(N,N- Dimethylamino)-4'- (N',N'- dimethylamino) benzophenone(DDBP), 4-(N-Ethyl-N,N- dimethylamino)-4'-(N'-methylamino) benzophenone(ED-MBP), 4-(N- Ethyl-N-methylamino) -4'-(N', N'-dimethylamino) benzophenone(EM-DBP) and 4-(N-Ethyl-N-methylamino)- 4'-(N'- methylamino) benzophenone(ED-BP).
Key words:  Shewanella oneidensis MR-1  methyl green  decolorization  product analysis  toxicity  biomechanism

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