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一起育苗场海带幼苗绿烂的病因分析
白露,阎永伟,王珊珊,李杰,张文彬,杨慧超,王朋梅,莫照兰
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(上海海洋大学,水产科学国家级实验教学示范中心,国家水生动物病原库,上海 201306; 中国水产科学研究院黄海水产研究所,青岛海洋科学与技术试点国家实验室,海洋渔业科学与食物产出过程功能实验室,农业农村部海水养殖病害防治重点实验室,青岛 266071;哈尔滨理工大学荣成校区,威海长青海洋科技有限公司,荣成 264300;上海海洋大学,水产科学国家级实验教学示范中心,国家水生动物病原库,上海 201306; 中国水产科学研究院黄海水产研究所,青岛海洋科学与技术试点国家实验室,海洋渔业科学与食物产出过程功能实验室,农业农村部海水养殖病害防治重点实验室,青岛 266071; 中国海洋大学海洋生命学院,青岛 266003)
摘要:
绿烂病是海带育苗期间常见的一种病害。2018年在山东一海带育苗场的调查中发现海带苗存在大面积绿烂现象。为了探析病害发生的原因,进行了育苗场水质分析、养殖模拟实验、褐藻酸降解菌分离鉴定和细菌回接感染,从环境因子和微生物相互作用角度进行了分析。水质分析结果显示,海带育苗池水的氮营养盐含量(30~35 mg·L-1)比育苗规程高近10倍左右,磷营养盐含量(2.1~2.2 mg·L-1)比海带育苗规程要求高5~6倍;养殖模拟实验显示,在10℃、光照2 000 ~ 4 000 lx、与育苗场近似的高营养盐浓度条件下,养殖的海带苗发生绿烂;从绿烂海带苗分离鉴定的附生优势菌属为YangiaGlaciecola;回接感染实验结果显示,在氮磷浓度偏高情况下,细菌Glaciecola.DH-2-3可以引起海带苗发生绿烂,Yangia.SJ-H-12未引起海带苗发生绿烂。根据这些结果,判断此次海带幼苗病烂是由高营养盐引起。
关键词:  海带  绿烂病  附生细菌  环境胁迫  病因分析
DOI:10.13610/j.cnki.1672-352x.20210112.012
基金项目:现代农业产业技术体系(CARS-50)资助。
Etiology analysis of green rot of kelp (Saccharina japonica) seedlings in a nursery farm
BAI Lu,YAN Yongwei,WANG Shanshan,LI Jie,ZHANG Wenbin,YANG Huichao,WANG Pengmei,MO Zhaolan
(National Demonstration Center for Experimental Fisheries Science Education, National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao 266071;Weihai Changqing Ocean Technology Co., Ltd., Rongcheng Campus, Harbin University of Science and Technology, Rongcheng 264300;National Demonstration Center for Experimental Fisheries Science Education, National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao 266071; College of Marine Life, Ocean University of China, Qingdao 266003)
Abstract:
Green rot is a common disease during kelp nursery. In 2018, it was found that kelp seedlings had a large area of green rot phenomenon through the investigation in a kelp nursery farm located in Shandong Province. To explore the causes of the disease, from the angle of interactions between environments and microorganisms, we conducted water quality analysis in the nurseries, breeding simulation experiments of seedlings, isolation and identification of alginic acid decomposing bacteria, and re-inoculation and infection assay. Water quality analysis showed that the content of nitrogen nutrients (30-35 mg·L-1) in the kelp nursery pond was approximately 10 times higher than the nursery protocol recommended, and the content of phosphorus nutrients (2.1-2.2 mg·L-1) was approximately 5-6 times higher than the nursery protocol recommended. Simulation experiments showed that green rot occurred to kelp seedlings under the condition of 10℃, 2 000 - 4 000 lx, and high nutrient concentration conditions, which were similar to nurseries in terms of temperature, illumination intensity and nutrient. The dominant epiphytic bacteria isolated from green rot kelp were affiliated to the genus Yangia and Glaciecola. Re-inoculation and infection of such strains demonstrated that bacteria Glaciecola.DH-2-3 was capable of causing green rot disease of kelp seedlings,
Key words:  Saccharina japonica  green rot disease  epiphytic bacteria  environmental stress  etiology analysis

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