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安农0711小麦品种3B染色体茎秆强度位点鉴定
张海萍,张钰岭,卢杰,王剑锋,闵晓宇,刘凯,韩雷锋,周燃,丁辉,司红起,常成,马传喜
0
(安徽农业大学农学院,农业部黄淮南部小麦生物学与遗传育种重点实验室,合肥 230036)
摘要:
倒伏是限制小麦产量进一步提升的主要因素之一,而茎秆强度是衡量小麦抗倒伏性的重要指标。为了鉴定控制茎秆强度基因位点,以安农0711(较强的茎秆强度和抗倒性)和河农825(较弱的茎秆强度和抗倒性)进行杂交构建的重组自交系群体为试验材料,于2017年测定其茎秆强度,利用SSR标记将控制小麦茎秆强度的位点(Qss.ahau-3B)初步定位于3B染色体上的wmc231-barc248标记之间,物理距离约为68~162 Mb。进一步利用小麦660K SNP芯片扫描两个亲本及高低池,并基于目标区段内差异的SNP,共开发了13个CAPS标记,将控制小麦茎秆强度的位点进一步缩短在EX-6650-barc248之间(150~162 Mb)。该研究结果为后续目标位点精细定位以及候选基因克隆提供了重要理论依据。
关键词:  小麦  倒伏  茎秆强度  SSR  CAPS
DOI:10.13610/j.cnki.1672-352x.20201113.002
投稿时间:2020-01-10
基金项目:国家现代小麦产业技术体系专项(CARS-03), 国家自然科学基金(31601298), 国家重点研发计划(2017YFD0100703), 安徽省科技重大专项(18030701182), 省级大学生创新创业计划(201810364100)和国家卓越农林人才教育培养计划改革试点项目“复合应用型人才培养”共同资助。
Identification of a locus for stem strength on chromosome 3B in Annong0711
ZHANG Haiping,ZHANG Yuling,LU Jie,WANG Jianfeng,MIN Xiaoyu,LIU Kai,HAN Leifeng,ZHOU Ran,DING Hui,SI Hongqi,CHANG Cheng,MA Chuanxi
(School of Agronomy, Anhui Agricultural University, Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry?of?Agriculture?and?Rural?Affairs, Hefei 230036)
Abstract:
Lodging is one of the main factors limiting the further improvement of wheat yield. Stem strength is an important indicator for determining wheat lodging resistance. In order to identify the locus for stem strength, in this paper, Annong0711 (stronger stem strength and lodging resistance) and Henong825 (weaker stem strength and lodging resistance) were used as parents to construct a RIL population and their stem strength phenotypes were determined in 2017. Using SSR markers, the locus controlling stem strength (designated Qss.ahau-3B) was preliminarily mapped in the interval of wmc231-barc248 on chromosome 3B, with the physical distance of 68-162 Mb. Thirteen CAPS markers were further developed to shorten the locus Qss.ahau-3B to Ex-6650-barc248 (150-162 Mb) based on the SNPs in the target region from the Wheat 660K Chip through scanning the two parents and two bulks. The results provide a useful theoretical basis for fine-mapping of the target locus and cloning of candidate genes.
Key words:  wheat  lodging  stem strength  SSR  CAPS

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