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促生细菌挥发性物质通过lncRNA调控拟南芥的 关键功能基因表达
李菲,石天龙,汤晓辛,龚记熠,徐小蓉,唐明
0
(西南喀斯特山地生物多样性保护国家林业局重点实验室,贵州师范大学生命科学学院,贵阳 550001; 贵州省植物生理与发育调控重点实验室,贵州师范大学生命科学学院,贵阳 550001)
摘要:
促生细菌的挥发性有机物可以促进拟南芥生长,提高拟南芥对多种生物和非生物胁迫的抗性。近年来的研究表明,植物lncRNA参与生长、发育和胁迫反应等生理过程。通过去rRNA全转录组测序,筛选出受到根际促生细菌ACCC 01380挥发性有机物处理影响的拟南芥lncRNA,并通过顺式分析获得可能受到这些lncRNA调控的蛋白编码基因;通过ceRNA分析获得lncRNA影响蛋白编码基因表达的调控网络。结果发现,拟南芥重要的生长发育调控和胁迫响应基因,如细胞分裂素降解基因AtCKX7、SOS2-like protein kinase PKS5,都受到促生细菌的挥发性有机物通过lncRNA的调控。研究结果初步证实了根际促生细菌挥发性有机物在促生过程中lncRNA的作用,并为进一步研究lncRNA功能以及根际促生细菌挥发性有机物的作用机理奠定了基础。
关键词:  枯草芽孢杆菌  拟南芥  挥发性有机物  lncRNA  ceRNA网络
DOI:10.13610/j.cnki.1672-352x.20200706.015
基金项目:国家自然科学基金委与贵州师喀斯特研究中心(U1812401)资助。
The regulation of growth promoting bacterial volatile substances on key functional genes in Arabidopsis thaliana through lncRNA network
LI Fei,SHI Tianlong,TANG Xiaoxin,GONG Jiyi,XU Xiaorong,TANG Ming
(Key Laboratory of Biodiversity Conservation of Southwest China Karst Mountain Area, School of Life Sciences, Guizhou Normal University, Guiyang 550001; Key Laboratory of Plant Physiology and Developmental Regulation, School of Life Sciences, Guizhou Normal University, Guiyang 550001)
Abstract:
Volatile organic compounds produced by plant growth promoting bacteria can promote the growth of Arabidopsis thaliana and improve its resistance to a series of biotic and abiotic stresses. Recent studies have shown that plant lncRNAs are involved in physiological processes such as growth, development and stress response. In this study, Arabidopsis lncRNAs affected by rhizosphere-promoting bacteria ACCC 01380 volatile organic compounds were screened using full transcriptome sequencing, and protein-coding genes that may be regulated by these lncRNAs were studied by ceRNA analysis. As results, we found that key growth regulation and stress response genes in Arabidopsis, such as cytokinin degradation gene AtCKX7, SOS2-like protein kinase PKS5, were regulated by the lncRNAs responsive to volatile organic compounds of ACCC 01380. This research will significantly improve our understanding of the role of lncRNAs in the physiological effects of rhizosphere-promoting bacterial volatile organic compounds and lay a foundation for the further study of lncRNAs function and the mechanism of bacterial volatile organic compounds.
Key words:  Bacillus subtilis  Arabidopsis thaliana  volatile organic compounds  lncRNA  ceRNA network

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