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基于微卫星标记的青弋江水系光唇鱼群体遗传变异
胡玉婷,段国庆,周华兴,凌俊,潘庭双,江河
0
(安徽省农业科学院水产研究所水产增养殖安徽省重点实验室,合肥 230031)
摘要:
为探讨长江支流青弋江水系光唇鱼的群体遗传变异,基于微卫星标记分析其5个采样点共105个样品的遗传多样性与遗传结构。光唇鱼5个群体均有较高的遗传多样性,其平均等位基因数(Na)为5.250~8.375、平均有效等位基因数(Ne)为3.580~4.415;平均观测杂合度(Ho)为0.618~0.677、平均期望杂合度(He)为0.669~0.774;平均多态信息含量(PIC)为0.629~0.699、平均Shannon多样性指数(I)为1.359~1.566。群体间Nei’s遗传距离(0.116~0.322)较小,群体遗传分化指数(FST)和分子方差分析(AMOVA)结果一致表明群体间无显著遗传分化。基于群体间Nei’s遗传距离构建的UPGMA系统进化树显示,秧溪河和浦溪河群体、麻川河和泾县群体分别聚为一支,最后与旌德群体聚在一起。这是由于群体间遗传距离与其栖息地之间距离远近显著相关。
关键词:  光唇鱼  青弋江  微卫星  遗传变异
DOI:10.13610/j.cnki.1672-352x.20200706.001
投稿时间:2019-10-24
基金项目:安徽省科技重大专项(17030701009), 安徽省农业科学院团队(2020YL038、2020YL039)和安徽省水产产业技术体系(皖农科[2016]84号)共同资助。
Population genetic variation of Acrossocheilus fasciatus in Qingyi River based on microsatellite markers
HU Yuting,DUAN Guoqing,ZHOU Huaxing,LING Jun,PAN Tingshuang,JIANG He
(Fishery Institute of Anhui Academy of Agricultural Sciences, Anhui Province Key Laboratory of Aquaculture & Stock Enhancement, Hefei 230031)
Abstract:
In the study, genetic diversity and genetic structure of 105 A. fasciatus samples collected from 5 localities in Qingyi River flowing into the Yangtze River were analyzed based on microsatellite markers. The allele number (Na) and the effective allele number (Ne) of the 5 populations ranged from 5.250 to 8.375 and from 3.580 to 4.415. The observed heterozygosity (Ho) was between 0.618-0.677 and the expected heterozygosity (He) was between 0.669-0.774. The range of average polymorphism information content (PIC) was 0.629-0.699 and Shannon diversity index (I) was 1.359-1.566. The above results indicated the high genetic diversity in all 5 populations. The results of Nei’s genetic distance, the genetic differentiation index (FST) and molecular variance analysis (AMOVA) revealed no significant genetic differentiations among populations. UPGMA trees among populations of A. fasciatus based on Nei’s genetic distance indicated that YX and PX population, MC and JX population, formed an clade, respectively. Then the 4 populations and JD population clustered together. The genetic distance among populations is significantly correlated with their geographic distance among different habitats.
Key words:  Acrossocheilus fasciatus  Qingyi River  microsatellite  genetic variation

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