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异源表达玉米ZmWRKY114基因增强拟南芥 对盐胁迫的敏感性
蔡荣号,李尉,陈浩伟,罗国伟,戴浩然,宋文雷,伯晨
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(安徽农业大学生命科学学院,合肥230036; 安徽农业大学作物抗逆育种与减灾国家地方联合工程实验室,合肥 230036)
摘要:
WRKY转录因子参与调控植物对各种非生物胁迫的应答反应。从玉米中分离得到一个响应高盐胁迫的WRKY基因,将其命名为ZmWRKY114。亚细胞定位实验表明ZmWRKY114定位于细胞核内且和ZmCaM2可以发生相互作用。为进一步研究ZmWRKY114基因的生物学功能,将ZmWRKY114基因构建到植物表达载体pCAMBIA1301上,并采用农杆菌转化法转化拟南芥,扩繁后对T3代转基因拟南芥进行了耐盐性试验。结果表明,转基因株系的绿苗率在高盐胁迫条件下显著低于野生型拟南芥,并且高盐胁迫对转基因拟南芥的主根抑制程度更大。同时,ZmWRKY114在拟南芥中的异源过表达还可以导致丙二醛的积累和相对电解质渗透率的增加,从而使转基因植物对盐胁迫的耐受性显著降低。上述结果表明ZmWRKY 114基因可能是盐胁迫反应的负调控因子。
关键词:  玉米  ZmWRKY 114基因  亚细胞定位  蛋白互作  盐胁迫
DOI:10.13610/j.cnki.1672-352x.20191030.001
基金项目:安徽省重大科技专项(18030701180), 国家自然科学基金(31801365)和中国博士后科学基金(2018M642507)共同资助。
The maize ZmWRKY114 gene negatively regulates salt stress tolerance in transgenic Arabidopsis
CAI Ronghao,LI Wei,CHEN Haowei,LUO Guowei,DAI Haoran,SONG Wenlei,BO Chen
(School of Life Science, Anhui Agricultural University, Hefei 230036; National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei 230036)
Abstract:
WRKY transcription factor is a large class of transcriptional regulatory proteins commonly found in higher plants. It can regulate the plant response to various abiotic stresses. In this study, we cloned and characterized the ZmWRKY114 gene from maize. ZmWRKY114 is a nuclear located protein and it can interact with ZmCaM2. Heterologous overexpression of ZmWRKY114 in Arabidopsis significantly reduced plant tolerance to NaCl stress, as determined by physiological indicator such as cotyledons greening rate, root growth, MDA content and relative electrolyte leakage. The above findings indicated that ZmWRKY 114 may function as a negative factor in plant response to salt stress.
Key words:  maize  ZmWRKY114 gene  subcellular localization  protein interaction  salt stress

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