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南林‘895’杂交杨组培苗对NaCl胁迫的生理响应
李翀,王杰,贾赵辉,程雪飞,彭孝楠,陈颖,张金池
0
(南方现代林业协同创新中心,江苏省水土保持与生态修复重点实验室,南京林业大学林学院,南京 210037;南京林业大学风景园林学院,南京 210037;南京林业大学生物与环境学院,南京 210037)
摘要:
以南林‘895’杂交杨( Populus delotides × P. euramericana cv. ‘Nanlin895’)组培苗为试验材料,采用CK、25、50、75和100 mmol·L-1 5种盐处理,研究盐胁迫对于‘895’杨叶片生理特性的影响。结果表明:(1) 25 mmol·L-1盐处理可以促进‘895’杨的生长,100 mmol·L-1盐处理抑制了‘895’杨的生长。(2)盐处理增加了过氧化氢(H2O2)与丙二醛(MDA)含量,在100 mmol·L-1时处理20 d时最为显著。(3)随着盐浓度的升高,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物(APX)呈先增加后下降的趋势,APX的峰值出现在25 mmol·L-1盐处理时,SOD、POD及CAT的峰值出现在50 mmol·L-1盐处理时。在100 mmol·L-1盐处理时,SOD、POD、CAT和APX活性显著低于对照。(4)随着盐浓度的升高,谷胱甘肽还原酶(GR)活性、还原型抗坏血酸(AsA)含量呈增加的趋势,100 mmol·L-1盐处理时,GR活性、AsA含量显著高于对照。谷胱甘肽(GSH)含量在1 d与10 d处理时随着盐浓度的上升而增加,20 d处理时,GSH含量在100 mmol·L-1盐处理较75 mmol·L-1盐处理微微下降。(5)根长、茎长和MDA、H2O2、GSH和AsA呈负相关关系;MDA、H2O2与SOD、CAT、APX呈负相关关系,与POD、GR呈正相关关系;SOD、CAT、APX3种酶互为正相关关系,POD与GR、SOD、CAT呈正相关关系。由此可见,低盐浓度(25 mmol·L-1)处理刺激了 ‘895’杨的生长,其通过提升抗氧化酶活性与抗氧化物质含量来维持活性氧平衡,避免受到毒害;高盐浓度(100 mmol·L-1)处理抑制了抗氧化酶与非酶抗氧化物质的作用,‘895’杂交杨受到了伤害;APX是 ‘895’杂交杨叶片内抵御膜脂过氧化最为关键的酶。
关键词:  盐胁迫  南林‘895’杨  组培苗  生理特性
DOI:10.13610/j.cnki.1672-352x.20200113.018
基金项目:林业公益性行业科研专项(201504406), 江苏省农业科技自主创新资金项目(CX(17)1004), 江苏省高校自然科 学研究重大项目(15KJA220004), 林业科学技术推广项目[2015]17号和江苏高校优势学科建设工程资助项目(PAPD)共同资助。
Physiological response of Populus delotides × P. euramericana cv.‘Nanlin895’ tissue culture seedlings to NaCl stress
LI Chong,WANG Jie,JIA Zhaohui,CHENG Xuefei,PEN Xiaonan,CHEN Ying,ZHANG Jinchi
(Jiangsu Province Key Laboratory of Soil and Water Conservation and Ecological Restoration, Co-innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037;College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037;College of Biology and Environment, Nanjing Forestry University, Nanjing 210037)
Abstract:
The antioxidant of Populus 'Nanlin895' seedlings were studied under salt stress of 0, 25, 50, 75 and 100 mmol·L-1, respectively. The results were as follows: (1) Salt treatment at the concentration of 25 mmol·L-1 promoted the growth of 'Nanlin895' poplar, while which inhibited its growth at 100 mmol·L-1. (2) The contents of H2O2 and MDA increased under the salt treatment, which were most significant when treated with 100 mmol·L-1 salt for 20 days. (3) With the increase of salt concentration, the peak value of APX appeared under 25 mmol·L-1 salt treatment, while the peak values of SOD, POD and CAT appeared under 50 mmol·L-1 salt treatment. The activities of these enzymes with 100 mmol·L-1 salt treatment were significantly lower than those in the control. (4) With the increase of salt concentration, the activity of GR and the content of AsA increased, which were significantly higher under 100 mmol·L-1 salt treatment than those in the control. The content of GSH increased with the increase of salt concentra-
Key words:  salinity stress  'Nanlin895' poplar  tissue culture seedling  physiological characteristics

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