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响应面法优化超声波提取滁菊水溶性多糖工艺 及其红外光谱分析
于士军,王睿,祁婉玲,张莹莹,盛琪,杜亚男,马若兰
0
(滁州学院生物与食品工程学院食品检验检测中心,滁州 239000)
摘要:
利用响应面法优化了超声波提取滁菊水溶性多糖的工艺条件。首先研究了超声波功率、提取温度、提取时间和料液比4个因素对滁菊水溶性多糖得率的影响,然后在单因素试验的基础上,通过响应面Box-Behnken实验设计与响应面分析对滁菊水溶性多糖的提取工艺进行了优化。得到最佳提取条件为超声波功率180 W、提取温度65℃、提取时间30 min、液料比40 mL·g-1。在此条件下滁菊水溶性多糖的提取得率为(8.22±0.57)%,与模型预测值7.94%较接近,经检测二者差异不显著,表明该模型是可靠的。体外抗氧化试验表明,在试验的0.25~4 mg·mL-1的范围内,滁菊水溶性多糖的还原力及对羟基自由基清、超氧自由基和DPPH自由基的清除能力随浓度的增加而增强。红外光谱分析表明,提取得到的滁菊多糖为α-型吡喃构成的酸性蛋白聚糖。
关键词:  滁菊  多糖  超声波  响应面  优化
DOI:10.13610/j.cnki.1672-352x.20191013.008
投稿时间:2018-09-19
基金项目:科技部星火计划项目(2015GA710033), 安徽省大学生创新训练项目(201710377065), 滁州学学院科研项目(2017PY04), 滁州学院大学生创新训练项目(2017CXXL071)和滁州学院生物与食品工程学院实验室开放课题(SWSP201604KF)共同资助。
Optimization of ultrasound extraction of water-soluble polysaccharides from Chrysanthemum morifolium (Ramat) cv. Chuju by response surface methodology and analysis of FTIR
YU Shijun,WANG Rui,QI Wanling,ZHANG Yingying,SHENG Qi,DU Yanan,MA Ruolan
(Food Inspection and Testing Center, School of Biology and Food Engineering, Chuzhou University, Chuzhou 239000)
Abstract:
In this work, the ultrasound extraction technology of water-soluble polysaccharides from Chrysanthemum morifolium (Ramat) cv. Chuju was optimized by response surface methodology. Firstly the effects of four independent factors, such as ultrasound power, extraction temperature, extraction time and the ratio of water to raw material, on the extraction yield of water-soluble polysaccharides were investigated, and then the extraction process was optimized by Box-Behnken experiment design (BBD) and response surface methodology (RSM). The results indicated that the optimal extraction conditions were as follows: ultrasound power, 180 W; extraction temperature, 65℃; extraction time, 30 min; the ratio of water to raw material, 40:1 (mL·g-1). Under these optimal conditions, the experimental yield of water-soluble polysaccharides was (8.22 ± 0.57)%, which was well matched with the value of 7.94% predicted by the model, indicating that the model is accurate and reliable. The results of antioxidant experiments in vitro indicated that the reducing power and the scavenging abilities of hydroxyl radicals, superoxide radicals and DPPH radicals increased with the increase of the concentration of the polysaccharides in the range of 0.25-4 mg·mL-1. FTIR analysis showed that the obtained polysaccharides was acid polysaccharides with protein and composed of α-configuration pyranose.
Key words:  Chrysanthemum morifolium  polysaccharide  ultrasound  response surface methodology  optimization

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