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茶树越冬芽萌展期碳氮代谢动态及相关基因分析
吴琼,刘丹丹,阮旭,沈季雪,徐奕鼎,王文杰,张正竹,夏恩华
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(安徽省农业科学院茶叶研究所,合肥 230031; 安徽农业大学省部共建茶树生物学与资源利用国家重点实验室,合肥 230036)
摘要:
以早生型茶树品种“皖茶4号”(原名红旗1号)为材料,研究茶树越冬腋芽(Axillary bud, AB)与毗邻成熟叶(下称母叶,Mature leaf,ML)萌展期的碳氮代谢动态变化,同时根据腋芽全转录组数据分析相关基因的表达情况。结果表明,不同发育时期的腋芽与母叶中的淀粉、可溶性糖、NSC及糖/淀粉比值差异极显著(P<0.01);腋芽与母叶的可溶性蛋白含量差异极显著(P<0.01);腋芽中SPS酶活性在3个发育时期差异极显著(P<0.01),母叶中SPS、ATP、ALT酶活性在不同发育时期差异极显著(P<0.01);通过比较腋芽各生育期的基因表达情况,发现有8个与碳氮代谢相关的基因表达差异显著(P<0.05)。
关键词:  茶树  越冬芽  碳氮代谢
DOI:10.13610/j.cnki.1672-352x.20191013.002
基金项目:安徽省农业科学院人才发展专项(17F0812), 安徽省重点研究与开发项目(1704a07020065), 安徽省农业科学院院所共建团队项目(18C0832)和现代农业产业技术体系建设专项资金项目(CARS-19)共同资助。
Analysis of dynamic changes of carbon and nitrogen metabolism and related gene expression in overwintering axillary bud during germination period of tea plant(Camellia sinensis)
WU Qiong,LIU Dandan,RUAN Xu,SHEN Jixue,XU Yiding,WANG Wenjie,ZHANG Zhengzhu,XIA Enhua
(Tea Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031; State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036)
Abstract:
The dynamic changes of carbon and nitrogen metabolism in overwintering axillary buds (AB) and adjacent mature leaves (ML) of tea plants during the germination period were detected using the early-generation tea tree variety “Wancha 4” (formerly known as “Hongqi 1”) as the material. The expression of related genes in axillary buds was also analyzed based on the total transcriptome data. The results showed that: from winter dormancy to bud flush stages, the ratios of starch, soluble sugar, NSC and sugar/starch in axillary buds and mature leaves at different developmental stages were extremely significantly different (P<0.01); the difference in soluble protein content between axillary buds and mature leaves was extremely significant (P<0.01); the activity of SPS in the three developmental stages was extremely significant (P<0.01); the activities of SPS, ATP and ALT in the mother leaves were significantly different at different developmental stages (P<0.01). It was found that there are significant differences in the expression of 8 genes involved in carbon and nitrogen metabolism (P<0.05).
Key words:  tea plant  overwintering axillary bud  carbon and nitrogen metabolism

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