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中老年期CD-1小鼠海马突触蛋白Arc含量改变 及其与认知功能下降的相关性研究
张折折,吴永芳,李学伟,曹磊,陈贵海,王芳
0
(安徽医科大学第一附属医院神经内科,合肥 230022;安徽医科大学附属巢湖医院神经内科(睡眠障碍科),合肥 238000;南华大学附属第一医院神经内科,衡阳 421001;安徽医科大学第二附属医院神经内科,合肥 230601)
摘要:
探究中老年CD-1小鼠海马突触蛋白Arc含量的改变及其与空间学习记忆能力下降的相关性。选取3月龄和15月龄CD-1小鼠各10只(雌雄各半),用Morris水迷宫和免疫组织化学法分别检测其空间学习记忆能力和海马Arc蛋白相对含量。与3月龄相比,15月龄鼠在Morris水迷宫学习期游泳路程延长(F(1,16)= 5.338, P<0.05),记忆期靶象限内游泳路程百分比下降(F(1,16)=78.013, P<0.01),海马CA1和CA3区Arc水平增加(P<0.05)。相关分析显示15月龄鼠海马CA1、CA3和DG区Arc相对含量与学习期游泳路程正相关(P<0.05),CA3、DG区Arc含量还与记忆期靶象限游泳路程百分比负相关(P<0.05)。中老年CD-1小鼠海马Arc含量呈亚区特异性增高,可能涉及其空间学习记忆损害。
关键词:  小鼠  衰老  Arc蛋白  学习记忆
DOI:10.13610/j.cnki.1672-352x.20190716.005
基金项目:国家自然科学基金(81671316), 国家自然科学基金青年基金(81301094)和安徽省自然科学青年基金(1708085QH182)共同资助。
Change of synaptic protein Arc in hippocampus and its correlation with cognitive impairment in middle-aged CD-1 mice
ZHANG Zhezhe,WU Yongfang,LI Xuewei,CAO Lei,CHEN Guihai,WANG Fang
(Department of Neurology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022;Department of Sleep Disorders, the Affiliated Chaohu Hospital of Anhui Medical University, Hefei 238000;Department of Neurology, the First Affiliated Hospital of Nanhua University, Hengyang 421001;Department of Neurology, the Second Affiliated Hospital of Anhui Medical University, Hefei 230601)
Abstract:
This study explored the effect of Arc and its correlation with the impairment of spatial learning and memory in aged CD-1 mice. Ten CD-1 mice (5 males and 5 females) were selected from 3- and 15-month old respectively. The abilities of spatial learning and memory were accessed with Morris water maze (MWM), and the relative content of synaptic protein Arc in hippocampus were detected using immunohistochemical method. The results indicated that the swimming distance during the learning phase was significantly longer and the percentage of distance during the probe phase was significantly less in the 15-month mice than those in the 3-month old mice (F(1,16)= 5.338, P < 0.05; F(1,16)=78.013, P < 0.01). The 15-month old mice had significantly higher Arc in the CA1 and CA3 subregions of hippocampus than the 3-month mice (P < 0.05). In the mice aged at 15-month old, the Arc level in the CA1, CA3 and DG positively correlated with the learning swimming distance (P < 0.05), and the DG and CA3 negatively correlated with the probe percentage of distance within the target quadrant (P < 0.05). Our findings suggested that the Arc level of hippocampus increased in a subregion-specific manner, which might be involved in the impairment of spatial learning and memory in middle-aged CD-1 mice.
Key words:  mouse  aging  Arc  learning and memory

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