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| 皖浙两省宠物犬海氏哈曼德(Hammondia heydorni) 球虫的分子鉴定 |
| 陈苗,韩枫,张衡,阚珍珍,唐莉,李慧,刘欣超,李文超,顾有方 |
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| (安徽科技学院动物科学学院，凤阳 233100;太和县发展和改革委员会，阜阳 236600) |
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| 摘要: |
| 确定皖浙两省宠物犬海氏哈曼德（Hammondia heydorni）球虫的感染情况。从安徽多地和浙江杭州共采集315份新鲜宠物犬粪样，提取粪便样本基因组DNA，所有样品先采用基于海氏哈曼德球虫/犬神经孢子虫 （H. heydorni/N. caninum）ITS1 基因的通用引物进行PCR检测，对获得的阳性扩增产物进行测序和分析，随后对阳性样品用海氏哈曼德球虫的特异性PCR引物进行扩增验证。PCR检测及序列分析发现315份粪便样本中有2个海氏哈曼德球虫阳性样本，其自然感染率为0.63%。皖浙两省宠物犬有海氏哈曼德球虫感染，但感染率低。 |
| 关键词: 海氏哈德曼球虫 犬 分子鉴定 安徽 浙江 |
| DOI：10.13610/j.cnki.1672-352x.20190619.002 |
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| 基金项目:安徽省自然科学基金项目（1808085MC84）, 安徽省科技重大专项（16030701066）, 安徽省教育厅项目（KJ2014ZD09）, 安徽科技学院重点学科项目（AKZDXK2015A04）和安徽科技学院校级稳定人才项目（dkwd201702）共同资助。 |
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| Molecular identification of Hammondia heydorni in dogs in selected areas of Anhui and Zhejiang Provinces |
| CHEN Miao,HAN Feng,ZHANG Heng,KAN Zhenzhen,TANG Li,LI Hui,LIU Xinchao,LI Wenchao,GU Youfang |
| (College of Animal Science, Anhui Science and Technology University, Fengyang 233100;Taihe County Development and Reform Commission, Fuyang 236600) |
| Abstract: |
| To determine the prevalence of H. heydorni infection in pet dogs , a total of 315 fresh fecal samples were collected from pet dogs in Anhui and Zhejiang Province. All specimens were screened firstly by nested-PCR targeting H. heydorni/N. caninum ITS1 gene, and the positive amplified products obtained were sequenced and analyzed. Subsequently, the H. heydorni/N. caninum-positive samples were confirmed by H. heydorni ITS1 PCR. PCR and sequence analysis showed that 2 positive samples were found in 315 fresh fecal sample, and the prevalence for H. heydorni was 0.63%. The present study showed that the prevalence of H. heydorni was not high in the pet dogs from Anhui and Zhejiang Provinces. |
| Key words: Hammondia heydorni dog molecular identification Anhui Zhejiang |