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富含氨基的聚酰胺-胺树枝状大分子的合成与表征
徐珍霞,孙程,陈福生,吴仁蔚
0
(华中农业大学食品科学技术学院,武汉 430070)
摘要:
以乙二胺(EDA)和丙烯酸甲酯(MA)为原料,采用发散法合成了0.5代到3.0代聚酰胺-胺(PAMAM)树枝状大分子。分别考察了反应温度、反应时间对半代及整代PAMAM树枝状大分子合成工艺的影响,并对合成的PAMAM产物进行了傅里叶变换红外光谱(FTIR)、高效液相色谱(HPLC)表征及端基滴定分析。实验结果表明,生成PAMAM半代及整代产物的最适反应温度均为25 ℃,反应时间为24 h;采用该方法合成的3.0G PAMAM树枝状大分子的纯度为88.22%,表面伯胺数约为16个,与理论值一致。合成的PAMAM树状分子表层富含大量的氨基基团,可以与抗体、核酸适体等功能分子结合而应用于食品安全检测和医药领域。
关键词:  树枝状大分子  聚酰胺-胺  合成  表征
DOI:10.13610/j.cnki.1672-352x.20180825.027
投稿时间:2017-12-22
基金项目:国家自然科学基金项目(31201374)和中央高校基本科研业务费专项资金(2662015PY152)共同资助。
Synthesis and characterization of polyamidoamine dendrimers with amine groups
XU Zhenxia,SUN Cheng,CHEN Fusheng,WU Renwei
(College of Food Science & Technology, Huazhong Agricultural University, Wuhan 430070)
Abstract:
Polyamidoamine (PAMAM) dendrimers were synthesized by divergent method using ethylenediamine (EDA) and methyl acrylate (MA) as raw materials. The effects of reaction temperature and reaction time on the synthesis of half-generation and full-generation PAMAM dendrimers were investigated. PAMAM was characterized by FTIR, HPLC and end-group titration analysis. The experimental results showed that the optimum reaction temperature and reaction time was 25℃ and 24 h, respectively. The purity of 3.0G PAMAM dendrimers synthesized by this method was 88.22%. And the number of primary amines of 3.0G PAMAM was about 16, which is consistent with the theoretical value. A large number of amino groups of PAMAM dendrimers can be combined with antibodies, aptamers and other functional molecules for food safety testing and medicinal field.
Key words:  dendrimer  polyamidoamine  synthesis  characterization

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