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重金属镉胁迫下臭牡丹的生理响应及富集研究
张建新,张婷婷,龙昌莲,崔乐怡,张航,苟秋,吴玉环,刘鹏
0
(丽水职业技术学院,丽水 323000;浙江师范大学生态学研究所,金华 321004; 浙江师范大学植物学实验室,金华 321004;杭州师范大学生命与环境科学学院,杭州 310036)
摘要:
为探究镉胁迫对臭牡丹生理响应及各部位镉富集特征的影响,进行盆栽试验,设置不同浓度(0.00、0.10、0.30、0.60和0.90 mmol·L-1)镉梯度,测定12、24和36 d后植物抗氧化酶活性、叶绿素含量、叶绿素荧光参数及各部位镉含量。结果表明,SOD、CAT活性随胁迫浓度增加总体先升后降,POD活性在高浓度下变化显著,前期增强,后期减弱;短时间、低浓度的镉污染会刺激臭牡丹提高叶绿素a、叶绿素b、叶绿素总量和叶绿素a/b以适应不良环境,长时间、高浓度的胁迫则产生较强的抑制效应;叶片Fv/Fm在高浓度后期明显减小,ETR先升后降;臭牡丹各器官镉富集能力存在显著差异,表现为根>茎>叶,其富集系数与各部位镉富集量呈负相关,表明胁迫加深会阻碍根部镉富集过程从而产生毒害效果。
关键词:  臭牡丹  生理变化  镉富集  抗逆性
DOI:10.13610/j.cnki.1672-352x.20180825.007
基金项目:国家自然科学基金 (41571049, 41461010), 浙江省公益技术应用研究计划项目(2015C32127)和丽水市重点科技创新团队(2012cxtd08)共同资助。
Physiological response and bioconcentration of Clerodendrum bungei Steud.to cadmium
ZHANG Jianxin,ZHANG Tingting,LONG Changlian,CUI Leyi,ZHANG Hang,GOU Qiu,WU Yuhuan,LIU Peng
(Lishui Vocational and Technical College, Lishui 323000;Key Laboratory of Botany, Zhejiang Normal University, Jinhua 321004; Research Institute of Ecology, Zhejiang Normal University, Jinhua 321004;School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036)
Abstract:
To determine the effect of cadmium on the physiological response and enrichment characteristic of Clerodendrum bungei seedlings, different concentrations of cadmium (0.00, 0.10, 0.30, 0.60 and 0.90 mmol·L-1) were sprayed on Clerodendrum bungei leaves. The antioxidant enzyme activity, chlorophyll content, chlorophyll fluorescence parameters, and cadmium content in the leaves, roots, and stems were measured on the 12th day, 24th day and 36th day after cadmium treatment. The results showed that cadmium had different effects on perosidase (POD), superoxide dismutase (SOD), and catalse (CAT). The activities of SOD and CAT generally rose firstly and then fell with the increase of the stress concentration of cadmium, while POD activity changed significantly at high concentrations, increasing fristly and then fell. Short-term and low-concentration cadmium treatment improved the contents of chlorophyll a, chlorophyll b, total chlorophyll, and chlorophyll a/b for adapting to the adverse environment, while long-term and high-concentration stress had a strong inhibitory effect. Leaves’ Fv/Fm significantly declined in the late period under high-cadmium pollution, while photosynthetic electron transfer rate (ETR) firstly rose and then dropped. There were significant differences in enrichment ability of cadmium among organs of Clerodendrum bungei, with the strongest in roots and the weakest in leaves. Bioconcentration factors were negatively correlated with enrichment amount of Cd in different organs, suggesting deepened stress could hinder the enrichment of Cd in the roots and thus produced toxic effect.
Key words:  Clerodendrum bungei Steud.  physiological changes  cadmium enrichment  resistance against stress

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