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耐铅微生物的筛选及其吸附性
李同灵,黄寒娟,彭漪,王芳,张硕,祁伟亮,唐赟,刘松青
0
(西华师范大学生命科学学院,南充 637002; 成都师范学院化学与生命科学学院,成都 611130;西华师范大学生命科学学院,南充 637002; 四川农业大学资源学院,成都 611130)
摘要:
为从重金属污染的土壤中筛选出耐铅微生物,为土壤重金属生物修复提供参考,用Pb2+浓度梯度筛选培养法得到耐铅菌株,16S rRNA测序以及系统发育树初步鉴定菌株。在不同温度、盐浓度、pH梯度下研究耐铅菌株的耐受性。多个Pb2+浓度探究菌株在不同Pb2+浓度下的吸附性。结果表明,通过牛肉膏蛋白胨培养基培养,得到一株能够耐铅离子浓度在1 200 mg·L-1的菌株。16S rRNA基因序列系统发育树显示,菌株P15属于大肠杆菌属(Escherichia)。该菌株在铅离子浓度为200 mg·L-1下的去除铅离子能力最强,达到80%。 经过测定菌株P15的各项生理指标表明,菌株适宜的环境条件分别为温度30℃,pH6,盐浓度0.005 mg·L-1。该铅耐受性菌株P15在生物修复重金属污染的土壤上有较高的潜在应用价值。
关键词:  土壤  重金属  铅污染  吸附  耐铅细菌
DOI:10.13610/j.cnki.1672-352x.20180825.025
基金项目:四川省科技厅科研项目(2016NFP0091)资助。
Lead resistance of microbial screening and preliminary determination of its adsorption
LI Tongling,HUANG hanjuan,PENG Yi,WANG Fang,ZHANG Shuo,QI Weiliang,TANG Yun,LIU Songqing
(College of Life Science, China West Normal University, Nanchong 637002; College of Chemistry and Life Sciences, Chengdu Normal University, Chengdu 611130;College of Life Science, China West Normal University, Nanchong 637002; College of Resources, Sichuan Agricultural University, Chengdu 611130)
Abstract:
In order to screen lead-tolerant microorganisms from heavy metal contaminated soils for providing a reference for soil heavy metal bioremediation, using Pb2+ concentration gradient cultivation, a lead-tolerant strain was obtained, and further identified by 16S rRNA sequencing and phylogenetic tree. The tolerance of the lead-tolerant strain P15 was studied at different temperatures, salt concentrations and pH gradients, to explore the adsorbability of bacterial strain P15 in different lead concentrations. As results, the strain accordingly originating from lead 1 200 mg·L-1 was isolated with a series of beef peptone mediums of different lead concentrations. And 16S rRNA gene sequence phylogenetic tree showed that strain P15 belongs to Escherichia. The result showed that the Pb2+ adsorption rate of P15 was 80% in lead 200 mg·L-1. After the determination of the physiological indexes of strain P15, it was shown that the optimum environmental conditions were 30℃, pH6, and the best salt concentration of 0.005 mg·L-1. In conclusion, it is expected that P15 is a suitable bio-sorbent for lead in the soil.
Key words:  soil  heavy metal  lead pollution  adsorption  lead tolerant bacteria

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