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克氏原螯虾布氏柠檬酸杆菌的分离鉴定及药敏分析
朱若林,杨彩桥,蒋书东,沈娇娇,张晓华,鲍传和,唐绍帅
0
(安徽农业大学动物科技学院,合肥 230036)
摘要:
从患病的克氏原鳌虾(Procambarus clarkii)中分离纯化得到一株优势菌株AX1707。通过革兰氏染色、氧化酶试验、生化鉴定、16S rRNA基因测序和进化树构建等操作进行分析,结果显示,AX1707为布氏柠檬酸杆菌(Citrobter braakii),人工感染试验结果显示该菌具有较强致病性,药敏试验结果显示其对恩诺沙星、氧氟沙星、氟苯尼考、复方新诺明、庆大霉素和多西环素敏感,对四环素和新霉素中度敏感,对红霉素和氨苄西林耐药。
关键词:  克氏原鳌虾  布氏柠檬酸杆菌  分离鉴定  药敏试验
DOI:10.13610/j.cnki.1672-352x.20180825.029
投稿时间:2017-10-02
基金项目:国家自然科学基金青年项目(31402331)和安徽省现代农业产业技术体系(2016-2020)水产产业体系(皖农科[2016]84号)共同资助。
Isolation, identification and antibiotic sensitivity analysis of Citrobter braakii from Procambarus clarkii
ZHU Ruolin,YANG Caiqiao,JIANG Shudong,SHEN Jiaojiao,ZHANG Xiaohua,BAO Chuanhe,TANG Shaoshuai
(School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036)
Abstract:
One predominant bacteria strain named AX1707 was isolated and purified from diseased Procambarus clarkii, which was further identified as Citrobter braakii by means of gram stain, oxidase test, biochemical identification, sequencing of 16S rRNA gene and phylogenetic analysis. Artifical infection proved that AX1707 had strong pathogenicity to healthy Procambarus clarkii. The antibiotic sensitivity test showed that AX1707 was sensitive to enrofloxacin, ofloxacin, TMP/SMZ, gentamicin and doxycycline, and intermediate sensitive to tetracycline and fradiomycin, while it was resistance to erythromycin and ampicillin.
Key words:  Procambarus clarkia   Citrobter braakii  isolation and identification  antibiotic sensitivity test

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