| 摘要: |
| 以巢湖打捞的新鲜蓝藻藻泥为处理对象，利用冻融破壁的方式获得藻蓝蛋白（phycocyanin，PC）粗提液。采取两步盐析初步纯化粗提液获得PC，再用纤维素（Cellufine A-500）阴离子交换树脂中度纯化PC，最后用羟基磷灰石（HA）柱高度纯化PC。结果表明，在室温25℃下，两步盐析(NH4)2SO4的最佳摩尔浓度分别为1.0 mol·L-1和1.8 mol·L-1，PC纯度（A620/A280）达到2.168，PC回收率可达37.5%。再经Cellufine A-500和HA两步柱层析纯化，PC纯度（A620/A280）分别达到3.211和4.133，回收率分别为20.3%和7.2%，纯度达到试剂级纯度要求。 |
| 关键词: 藻蓝蛋白 两步盐析 阴离子交换 羟基磷灰石 |
| DOI：10.13610/j.cnki.1672-352x.20180620.009 |
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| 基金项目:国家“十二五”重大科技专项项目（2012ZX07103-004）资助。 |
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| Purification of phycocyanin from Chaohu cyanobacteria by fractional salting out and two steps column chromatography |
| SU Yu,ZHANG Fayu,YU Jinwei,WANG Jiaquan,XU Zimu |
| (School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009;School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009; Hefei Dongfangmeijie Molecular Materials Technology Co.Ltd., Hefei 230009) |
| Abstract: |
| The fresh algae mud from Chaohu was used to obtain the crude extract of phycocyanin (PC) using freezing and thawing. The crude extract was purified by two-step salting-out, and the PC was purified by cellulose (Cellufine A-500) anion exchange resin and finally purified by hydroxyapatite (HA) column. The results showed that At room temperature (25℃), the optimum molar concentrations of two-step salting-out (NH4)2SO4 were 1.0 mol·L-1 and 1.8 mol·L-1, respectively. The PC purity (A620 / A280) reached to 2.168 and the PC recovery was 37.5 %. The purity of PC (A620 / A280) reached 3.211 and 4.133, respectively, and the recovery was 20.3% and 7.2%, respectively, which reaches the reagent grade purity. |
| Key words: phycocyanin two-step salt precipitation anion exchange hydroxyapatite |
