引用本文:[点击复制]
[点击复制]
【打印本页】 【在线阅读全文】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 47次   下载 65 本文二维码信息
码上扫一扫!
辣椒RWP-RK转录因子分析
李菲,龚记熠,何小红,张习敏,乙引
0
(贵州师范大学生命科学学院贵州省植物生理与发育调控重点实验室,贵阳 550001)
摘要:
RWP-RK基因家族是植物特有的转录因子家族。该家族在植物氮元素信号传导和配子体发育过程中起核心的调控作用。使用公开的中国重要栽培品种遵辣1号及其野生亲本墨西哥辣椒种“Chiltepin”的基因组序列,鉴定出辣椒基因组中含有RWP-RK结构域的基因,并进行系统进化、染色体定位、保守序列和可能功能等分析。遵辣一号基因组中与拟南芥氮代谢重要调控因子AtNLP7同源的是CaZunla01g000097、CaZunla01g004485和CaZunla00g000405。遵辣1号的2个基因Capana03g001990和Capana03g000787与拟南芥卵细胞形成相关转录因子AtRKD1AtRKD2AtRKD4同源。这些基因在栽培品种遵辣1号和其野生祖先品种基因组中保持了高度的保守性。此外,辣椒基因组中存在特有的RWP-RK转录因子亚家族。这一亚家族的蛋白质氨基酸序列中含有独特的保守序列。这些序列在拟南芥RWP-RK转录因子中并不存在。辣椒栽培品种遵辣1号中有在其野生祖先“Chiltepin”中不存在的RWP-RK转录因子基因。鉴定出了分别与拟南芥RWP-RK转录因子高度相似、存在重大差异以及辣椒野生种和栽培种之间存在差异的RWP-RK转录因子成员,为后期辣椒RWP-RK转录因子家族的克隆和功能研究提供了重要的信息。对这些基因进行功能研究,会极大的促进辣椒对氮元素匮乏环境响应以及辣椒配子体发育的分子机制研究。
关键词:  辣椒  RWP-RK转录因子  结构域  亚家族  氮元素
DOI:10.13610/j.cnki.1672-352x.20180302.005
投稿时间:2017-06-09
基金项目:贵州省科学技术基金(2017GZ66249)资助。
Analysis of pepper RWP-RK transcription factors
LI Fei,GONG Jiyi,HE Xiaohong,ZHANG Ximin,YI Yin
(Key Laboratory of Plant Physiology and Development Regulation of Guizhou Province, College of Life Sciences, Guizhou Normal University, Guiyang 550001)
Abstract:
The RWP-RK gene family is a plant-specific transcription factor family. RWP-RK transcription factors play central roles in regulation of plant nitrogen signal transduction and gametophyte development. Using the genomic sequences of "Capsicum annuum L. Zunla-1", an important pepper cultivar in China and its ancestor Mexican pepper "Chiltepin", we identified the genes containing the RWP-RK domain in these two pepper genomes. We also carried out phylogenetic, chromosome localization, conserved sequences and possible function analysis. In the genome of Capsicum annuum L. Zunla-1, CaZunla01g000097, CaZunla01g004485 and CaZunla00g000405 were homologous to the essential regulatory factor (AtNLP7) controlling the nitrogen metabolism in Arabidopsis thaliana. Capana03g001990 and Capana03g000787 were homologous to Arabidopsis thaliana egg formation related transcription factors AtRKD1, AtRKD2 and AtRKD4. These genes maintain a high degree of conservation between the cultivar Zunla-1 and its wild ancestor "Chiltepin". In addition, there is a unique RWP-RK transcription factor subfamily in the pepper genomes. This subfamily protein contains unique motifs that are not existed in the Arabidopsis RWP-RK transcription factors. There is also a RWP-RK transcription factor unique in Capsicum annuum L. Zunla-1, but not in the wild ancestor "Chiltepin". In this study, we identified RWP-RK transcription factors that are highly similar or different to Arabidopsis RWP-RK transcription factors. These identified factors showed significant differences between the wild and cultivars of pepper. The results provide important information for pepper RWP-RK genes cloning and functional analysis. Functional studies on these genes will greatly promote the study of the molecular mechanism of peppers in response to nitrogen deficiency and pepper gametophyte development.
Key words:  Capsicumspp.  RWP-RK transcription factor  protein domain  subfamily  nitrogen

用微信扫一扫

用微信扫一扫