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帕金森病模型大鼠脑内CDNF表达变化
张彤宇,薛莹,周鹏飞,潘士锋,邢华
0
(扬州大学兽医学院,扬州225009;扬州大学兽医学院,扬州225009; 江苏省动物重要疫病与人兽共患病防控协同创新中心,扬州 225009)
摘要:
为了探讨帕金森病(PD)发病过程中大脑多巴胺能神经营养因子(CDNF)表达的变化,以大鼠为模型,研究了帕金森病CDNF及相关基因的表达变化,从而为基因治疗帕金森病提供理论依据。实验采用单侧黑质内单点注射6-羟多巴胺的方法制备帕金森病大鼠模型;运用免疫组织化学、Western blot及Q-PCR方法检测CDNF和多巴胺合成限速酶—酪氨酸羟化酶(TH)在模型大鼠黑质中表达的变化。研究结果发现,PD模型大鼠脑黑质区CDNF和TH阳性细胞数明显减少;进一步研究表明,与正常大鼠相比,模型大鼠CDNF mRNA表达水平虽未见显著改变(P>0.05),但CDNF及TH蛋白表达极显著降低(P<0.01)。提示帕金森病的发病可能与黑质中CDNF和TH表达量降低,进而抑制多巴胺合成有关。
关键词:  大鼠  帕金森病  大脑多巴胺能神经营养因子  表达
DOI:10.13610/j.cnki.1672-352x.20180302.027
基金项目:国家自然科学基金(31172284)和江苏高校优势学科建设工程项目(PAPD)共同资助。
Expression of CDNF in the brain of the parkinson's disease rat ZHANG Tongyu,XUE Ying,ZHOU Pengfei,PAN Shifeng,XING Hua
(College of Veterinary Medicine,Yangzhou University,Yangzhou 225009;College of Veterinary Medicine,Yangzhou University,Yangzhou 225009; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou 225009)
Abstract:
In order to investigate the expression of CDNF in the process of Parkinson's disease (PD), we used rat as an experimental model to determine the expression of dopaminergic neurotrophic factor and its related genes in the brain of PD to to provide a theoretical basis for gene therapy of PD. The rat model of PD was prepared by single-point injection of 6-hydroxydopamine in unilateral substantia nigra. Immunohistochemistry, western blot and Q-PCR methods were used to detect the expression of CDNF and threonine-hydroxylase (TH) in the substantia nigra of the model rats. We found that the number of CDNF and TH positive cells in the substantia nigra of the model rats was significantly decreased. Further research showed that when compared with the normal control rats, CDNF mRNA expression showed no change in the model rats (P>0.05), while CDNF and TH protein expression were significantly reduced (P<0.01).These results suggested that the incidence of PD may be relevant to the decreased expression of CDNF and TH in the substantia nigra, which further inhibit dopamine synthesis.
Key words:  rat  Parkinson's disease  cerebral dopamine neurotrophic factor  expression

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