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杂交榛子愈伤组织的诱导
汪青,丁之恩,常慧,许睿洁,周天驹,孙雨薇
0
(安徽农业大学茶与食品科技学院,合肥 230036)
摘要:
利用组织培养技术对杂交榛子无性器官进行培养研究,从而为后续人工控制条件下获得更多的紫杉醇提供基础性研究材料。结果表明,对于10年生杂交榛子的根、茎段和叶片3种外植体进行愈伤组织诱导,其中茎段愈伤组织诱导率最高为66%,基础培养基MS为最适培养基,最适生长调节剂组合为2,4-D 2.5 mg·L-1+6-BA0.5 mg·L-1
关键词:  杂交榛子  愈伤组织  诱导  诱导率
DOI:10.13610/j.cnki.1672-352x.20171214.024
投稿时间:2016-08-18
基金项目:安徽省林业厅世界银行五期项目“经济林混交林高效栽培及资源综合利用”资助。
Callus induction of cross-fibert
WANG Qing,DING Zhien,CHANG Hui,XU Ruijie,ZHOU Tianju,SUN Yuwei
(School of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036)
Abstract:
In this study, vegetative tissues of cross-fibert were cultivated using tissue culture technology, which can provide a basic research for subsequently gaining more paclitaxel materials. The results indicated that in three explants (root, stem, and leaf) of a ten-year-old cross-fibert, the stem section had the highest callus induction rate, reaching to 66%. The best basal medium was MS and the optimal plant growth regulators were the combination of 2,4-D 2.5 mg·L-1+6-BA 0.5 mg·L-1.
Key words:  cross-fibert  callus  induction  induction rate

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