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响应面法对绿色木霉产纤维素酶固态发酵条件优化
刘华,李崇高,黄建初
0
(广州工商学院,广州 510850;广州城市职业学院,广州 510000)
摘要:
为探讨绿色木霉固态发酵产生纤维素酶的最佳发酵条件,在单因素分析的基础上,采用Box-Benhnken方法设计实验,选取麸皮与秸秆粉质量比、培养基含水量和初始pH值作为影响因素,以产酶量为响应值建立二次回归方程,并通过响应曲面分析法分析数据并确定优化条件。结果显示,在不同条件下绿色木霉产纤维素酶活力存在显著差异(P<0.05),采用响应面法在培养温度29℃,硫酸铵添加量为2%时,获得了最适培养基成分为麸皮秸秆粉比例1.37:1,含水率250%(10 g干基),初始pH6.04,在72 h获得了最大产酶量,酶活为59.72 U·g-1,与基础培养基相比有近20%的提高。
关键词:  纤维素酶  绿色木霉  响应曲面法  固体发酵
DOI:10.13610/j.cnki.1672-352x.20171214.016
基金项目:浙江省重大科技专项(2017C02037)和安徽特色产业发展项目(皖财农[2016]188号)共同资助。
Condition optimization of solid fermentation for cellulase production by Trichoderma viride using response surface methodology
LIU Hua,LI Chonggao,HUANG Jianchu
(Guangzhou College of Technology and Business, Guangzhou 510850;Guangzhou City Polytechnic, Guangzhou 510000)
Abstract:
The aim of the study was to investigate the optimal fermentation conditions for cellulase production through solid state fermentation of Trichoderma viride. The study was designed based on the basis of single factor analysis. An orthogonal test was conducted by chosen three parameters, including the ratio of wheat bran to straw powder, moisture content and initial pH value as effect factors with the Box-Benhnken method. The quadratic regression equation was established based on the yield of enzyme, and the optimal conditions were determined using response surface methodology. The activity of Trichoderma viride cellulase displayed significant differences under different conditions (P<0.05). The optimal fermentation medium obtained when 2% ammonium sulfate was added at the culture temperature of 29 ℃ was as follows: wheat bran to straw powder ratio of 1.37:1, water content of 250% (10 g dry basis), and the initial pH value of 6.04. The highest enzyme activity of 59.72 U·g-1 (dry media) was obtained after 72 hours, which increased about 20% compared to that of the basal medium.
Key words:  cellulase  Trichoderma viride  response surface methodology  solid state fermentation

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