引用本文:[点击复制]
[点击复制]
【打印本页】 【在线阅读全文】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 148次   下载 339 本文二维码信息
码上扫一扫!
版纳微型猪近交系AO血型基因外显子7 和外显子8遗传特征分析
潘伟荣,查星琴,霍金龙,卿玉波,肖晶,魏红江,曾养志,成文敏
0
(云南农业大学动物科学技术学院,昆明 650201; 云南省版纳微型猪近交系重点实验室,昆明 650201)
摘要:
猪的UDP-N-乙酰半乳糖胺转移酶(UDP-N-acetylgalactosamine transferase,UDP-GalNAc)是负责转移一分子的N-乙酰-D-半乳糖胺基(NAcGa1)到H底物上形成A抗原,从而决定A型血的转移酶,AO血型系统的A基因编码A转移酶。采用PCR技术和直接测序的方法对版纳微型猪近交系UDP-GalNAc基因外显子7和外显子8进行多态性分析,均仅检测到1种基因型。通过序列比较发现,版纳微型猪近交系UDP-GalNAc基因外显子7和外显子8与NCBI 数据库中14个其他物种的ABO血型基因存在较高的同源性。与人相比,猪外显子7第92~97位缺失编码两个氨基酸的6个碱基。构建的基因进化树分析结果显示版纳微型猪近交系与牛、羊、鲸亲缘关系最近。
关键词:  UDP-N-乙酰半乳糖胺转移酶基因  版纳微型猪近交系  外显子7和8  PCR  序列分析
DOI:10.13610/j.cnki.1672-352x.20170811.017
基金项目:云南省教育厅科学研究基金项目(2014Z077)资助。
Genetic analysis of exon 7 and exon 8 of the UDP-GalNAc gene in Banna Mini-pig inbred line
PAN Weirong,ZHA Xingqin,HUO Jinlong,QING Yubo,XIAO Jin,WEI Hongjiang,ZENG Yangzhi,CHENG Wenmin
(College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201; Key Laboratory of Banna Mini-pig Inbred Line of Yunnan Province, Kunming 650201)
Abstract:
The porcine UDP-N-acetylgalactosamine transferase (UDP-GalNAc) is the enzyme responsible for transfering a molecule of N-acetylgalactosamine (NAcGa1) to the H substrate to synthesize A antigen, which determines A group, the blood group A gene encoding A transferase. In this study, we PCR amplified the exon 7 and exon 8 sequences of the UDP-GalNAc gene (134 bp and 341 bp in length, respectively) in Banna Mini-pig inbred line. Sequencing of both the exons yielded just one single genotype in the tested population. Homology search in NCBI database with 14 species revealed that the similarity varied from 100 % (pig) to 72.6 % (mice). We documented a deletion of six bases (from the 92th to the 97th) in exon7 in Banna Mini-pig when compared to human, which resulted in missing of two amino acids. Phylogenetic analysis with 14 selected species revealed that Banna Mini-pig shared the same genetic cluster with cattle, sheep, goat and minke whale.
Key words:  UDP-N-acetylgalactosamine transferase gene  Banna Mini-pig Inbred Line  exon 7 and 8  PCR (polymerase chain reaction)  sequence analysis

用微信扫一扫

用微信扫一扫