| 摘要: |
| 利用实验室筛选得到的一株枯草芽孢杆菌发酵生产γ-聚谷氨酸（γ-PGA），主要对其发酵工艺参数进行了优化。先通过单因素优化及PB试验筛选重要因子，然后利用Box-Behnken优化发酵工艺最佳组合，获得了最佳培养基组成和最佳培养条件，且在摇床水平上使γ-PGA产量由最初的6.03 g·L-1提高到10.98 g·L-1。接着通过对补料工艺的探索，确定了最佳补料时间和补料配方，最后在5 L发酵罐上进行了放大生产，最终γ-PGA的最高产量可达到31.18 g·L-1。 |
| 关键词: 枯草芽孢杆菌 γ-PGA 发酵工艺优化 |
| DOI：10.13610/j.cnki.1672-352x.20170811.020 |
| 投稿时间：2016-12-26 |
| 基金项目:江苏省科技厅产学研联合创新资金“前瞻性研究项目（BY2013001-03）”资助。 |
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| Production of poly-γ-glutamic acid by fermentation of Bacillus subtilis |
| SANG Xiumei,WANG Jing,ZHAO Jie,GAO Pin,LIU Ping,YIN Zhimin |
| (School of Life Sciences, Nanjing Normal University, Institute of Biochemistry and Biological Products, Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Nanjing 210046) |
| Abstract: |
| In this study, we optimized the fermentation parameters of poly-γ-glutamic acid produced by Bacillus subtilis, a strain selected through a lab screen. A single factor experiment and PB experiment were first carried out, and then the optimal composition of the medium and optimum culture conditions were obtained by optimizing the fermentation process with Box-Behnken. The yield of γ-PGA increased from 6.03 g·L-1 to 10.98 g·L-1 at the shaker level. We further explored the feeding process to determine the best time and feeding materials. Finally, a 5 L fermentor was used to increase the production, and the highest yield of γ-PGA reached to 31.18 g·L-1. |
| Key words: Bacillus subtilis γ - PGA optimization of fermentation process |