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2,4-表油菜素内酯(EBR)对菜用甘薯抗寒生理生化的影响
张小贝,祝志欣,南文卓,孙言博,朱国鹏
0
(海南大学热带作物种质资源保护与开发利用教育部重点实验室/热带农林学院,海口 570228)
摘要:
菜用甘薯抗寒性能是其在早春及秋冬季作为蔬菜供应的重要制约因素。以徐菜薯1号菜用甘薯为实验材料,通过对甘薯叶进行外源2,4-表油菜素内酯(EBR)喷施处理,探究其对菜用甘薯抗寒生理生化的影响;并对其诱导抗寒的机理进行研究。结果表明,0.1 mg·L-1的EBR处理下甘薯的抗寒性能最佳。与对照相比在8℃低温胁迫下的48 h内,甘薯叶中3种抗氧化酶(CAT、POD和SOD)活性明显高于对照,3种渗透调节物质(可溶性蛋白质、可溶性糖和脯氨酸)中可溶性蛋白的含量有大幅提高,而细胞的损伤指标(电导率和MDA)的数值均低于对照。外源EBR处理提高了甘薯叶中抗氧化酶的活性及渗透调节物质的含量,减少了细胞膜的损伤,增强了菜用甘薯抵抗低温胁迫的能力。
关键词:  EBR  菜用甘薯  低温胁迫  抗寒生理生化
DOI:10.13610/j.cnki.1672-352x.20170524.027
投稿时间:2016-09-01
基金项目:热作无公害生产技术集成与示范推广(15RZNJ-57)和优质富硒菜用甘薯周年生产技术示范与推广(HNXH201530)共同资助。
Effects of 2, 4-epibrassinolide (EBR) on cold resistance-related physiological-biochemical indexes of vegetable sweet potato
ZHANG Xiaobei,ZHU Zhixin,NAN Wenzhuo,SUN Yanbo,ZHU Guopeng
(Ministry of Education Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources/ Institute of Tropical Agriculture and Forestry,Hainan University, Haikou 570228)
Abstract:
The cold intolerance greatly restricted the supply of vegetable sweet potato in early spring, autumn and winter. By using vegetable sweet potato ‘Xucai-1’, this research explored the effects of 2, 4-epibrassinolide (EBR) spraying treatment on the cold resistance-related physiological-biochemical indexes in sweet potato leaves under cold stress. Our results showed that spraying 0.1 mg·L-1 EBR was most effective. Within 48 hours of cold stress (8℃), the activities of three anti-oxidases (CAT, POD and SOD) were significantly higher than those of the control. Of three osmotic adjustment substances (soluble protein, soluble sugar and proline), the level of soluble protein had greatly increased and the relative electrical conductivity and MAD content which represented the extent of cellular damage were lower compared with the control. Overall, exogenous EBR treatment could increase the activity of anti-oxidases and the content of osmotic adjustment substances, and reduce the cell membrane damage of sweet potato leaves. Spraying of EBR could enhance the resistance of vegetable sweet potato to cold stress.
Key words:  EBR  vegetable sweet potato  cold stress  cold resistance-related physiological-biochemical indexes

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