引用本文:[点击复制]
[点击复制]
【打印本页】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览次   下载 本文二维码信息
码上扫一扫!
实时荧光定量PCR检测转基因玉米Bt176测量不确定度分析
熊娟,黄启红,伍玲燕,蔡大川,张志军,黄璇莹,陈敏儿
0
(中国广州分析测试中心,广东省化学危害应急检测技术重点实验室,广州 510070)
摘要:
应用实时定量PCR方法测定样品中转基因玉米Bt176的含量,并从多个方面来分析测量的不确定度。结果显示,A类不确定度为0.0003,B类不确定度为0.0007,合成不确定度为0.001,有效自由度veff为272,包含概率P为95%,扩展不确定度U=0.002,测量结果为(0.8±0.2)%。不确定度评估结果表明,测量不确定度的主要来源是微量液体转移量的偏差。
关键词:  转基因玉米Bt176  实时荧光定量PCR  定量检测  不确定度评估
DOI:10.13610/j.cnki.1672-352x.20170208.023
投稿时间:2016-03-04
基金项目:广东省省级科技计划项目(2014B070705001)和广东专业镇中小微企业食品安全与质量控制检测服务平台(2013B091604003)共同资助。
Uncertainty in detecting event-specific fragments of genetically modified maize BT176 by quantitative real time PCR
XIONG Juan,HUANG Qihong,WU Lingyan,CAI Dachuan,ZHANG Zhijun,HUANG Xuanying,CHEN Miner
(Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, China National Analytical Center, Guangzhou 510070)
Abstract:
The real-time fluorescent quantitative PCR method was used to detect the event-specific fragments in a sample of genetically modified maize BT176 and the uncertainty of measurement derived from several aspects was analyzed. The results showed that uncertainty of type A and type B was 0.0003 and 0.0007, respectively; the combined standard uncertainty was 0.001; the?effective?degree of freedom was 272; the coverage probability was 95%; the expanded uncertainty was 0.001, and the measured result was 0.8%±0.2%. This result indicated that the main source of uncertainty was the trace amount of liquid transfer, which should be paid more attention to in future detection.
Key words:  genetically modified maize Bt176  real-time fluorescent quantitative PCR method  quantitative detection  uncertainty evaluation

用微信扫一扫

用微信扫一扫