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纤维素酶Umcel9y-1在银杏叶总黄酮提取中的增效作用
王旭,王志,蔡梦宇,邬艳博,周育
0
(安徽农业大学茶树生物学与资源利用国家重点实验室,合肥 230036)
摘要:
选取银杏叶片为试验材料,研究纤维素酶Umcel9y-1在银杏叶片总黄酮提取中的应用价值。试验结果表明,0.08 mg·mL-1纤维素酶Umcel9y-1,在其最适反应条件下(pH7.0,37oC),处理银杏叶片1 h、2 h、4 h和过夜(12 h),总黄酮的提取量较对照组分别提高了20.3%、20.7%、16.8%和15.0%。纤维素酶试验组处理2 h,总黄酮的提取量可以达到对照组12 h提取所得总黄酮量的97.0%,纤维素酶Umcel9y-1显著缩短了银杏叶总黄酮提取时间。采用超高效液相色谱对2种提取方法获得的提取液进行药用成分检测,发现添加纤维素酶Umcel9y-1对中药提取液活性成分没有显著的影响。
关键词:  纤维素酶  银杏叶  总黄酮  提取
DOI:10.13610/j.cnki.1672-352x.20170208.024
投稿时间:2016-07-05
基金项目:安徽省自然科学基金 (1608085QC57)和安徽省知名专家 (1608085J08)共同资助。
Synergized action of cellulase Umcel9y-1 on the extract efficiency of total flavonoid from Ginkgo biloba L. leaves
WANG Xu,WANG Zhi,CAI Mengyu,WU Yanbo,ZHOU Yu
(State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036)
Abstract:
Ginkgo leaves were selected as experimental materials to investigate the cellulase Umcel9y-1 synergized action on total flavonoids extraction. Under the optimum reaction conditions (pH 7.0, 37 oC), the extraction yields of total flavonoids increased by 20.3%, 20.7 %, 16.8% and 15% of the corresponding control after the leaves were treated with 0.08 mg·mL-1 Umcel9y-1 for 1 h, 2 h, 4 h and overnight (12 h). The extraction yield of toal flavonoids in the 2-hour treatment group reached 97.01% of the overnight-treatment of the control. The results showed the extract efficiency was greatly improved by the addition of cellulase Umcel9y-1. Moreover, the medical ingredients of the extracts were evaluated by Ultra performance liquid chromatography (UPLC), indicating that Umcel9y-1 had no obvious influence on the medical ingredients of the extract compared to the corresponding control.
Key words:  cellulase   Ginkgo leaf  total flavonoid  extraction

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