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姜黄素修饰的纳米金棒抑制胃癌细胞SGC-7901的作用
孙冬冬,朱梦瑶,魏雨婷,王海军,汪维云
0
(安徽农业大学生命科学学院,合肥 230036)
摘要:
恶性肿瘤严重威胁着人类的健康。合成了姜黄素修饰的纳米金棒(Cur@Au NRs),并考察其对胃癌细胞(SGC-7901)的作用,采用MTT法测试其对5种肿瘤细胞株的体外增殖活性的影响,同时观察其对SGC-7901形态学的影响;利用实时xCELLigence细胞分析系统 (RTCA)检测不同浓度Cur@Au NRs对SGC-7901增殖能力及迁移能力的影响;利用划痕愈合实验及血管形成实验检测Cur@Au NRs对细胞的迁移及血管形成的抑制作用;采用腹腔注射Cur@Au NRs后与正常小鼠内脏组织切片的对比,对其进行毒理学研究。研究结果表明,Cur@Au NRs具有良好的抗肿瘤活性,尤其对SGC-7901,且其IC50值明显低于姜黄素,并且能够抑制SGC-7901的增殖和迁移。毒理学研究表明即使在高剂量下也不具有明显的毒性。
关键词:  姜黄素  纳米金棒  抗肿瘤  RTCA
DOI:10.13610/j.cnki.1672-352x.20161205.019
投稿时间:2016-08-10
基金项目:国家自然科学基金青年科学基金项目(21401002), 安徽省自然科学基金青年项目(1508085QB37)和安徽农业大学“大学生创新创业训练计划”创新训练项目经费共同资助。
The inhibitory effect of gold nanorods through reduction with curcumin on human gastric cancer?SGC-7901?cells
SUN Dongdong,ZHU Mengyao,WEI Yuting,WANG Haijun,WANG Weiyun
(School of Life Science, Anhui Agricultural University, Hefei 230036)
Abstract:
Malignancies?are?of the?most?common?illnesses?seriously?harmful?to?human health. In this study, gold nanorods were prepared from CTMAB capped Au seeds reduced with curcumin (Cur) to?investigate?the?effect?of Cur@Au NRs on human?gastric?cancer?SGC-7901?cells. The MTT method and optical microscope were used to evaluate the effect of Cur@Au NRs on the anti-proliferative activity of tumor cell lines in vitro and the morphology of gastric cancer cells was also observed. The replication of SGC-7901?cells was detected using the real-time cell assay (RTCA) system. The migration of cells and the angiogenesis inhibition of nano gold nanorods were detected using wound healing and blood vessel formation test. Tntraperitoneal injections of gold nanorods were used to study the toxicology. Preliminary results showed that Cur@Au NRs showed a potent growth inhibition against all tested cell lines, especially the human?gastric?cancer. The IC50 value of Cur@Au NRs was significantly lower than that of curcumin. Cur@Au NRs showed the inhibitory effect on the proliferation and migration of SGC-7901. The toxicology study showed Cur@Au NRs a negligible toxicity, even at a high dosage.
Key words:  curcumin  gold nanorods  antitumor  RTCA

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