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薄壳山核桃嫁接苗“小脚”现象的导管分子特性
孙志超,程慧,常爱玲,靡瑶琦,黄瑞敏,王正加
0
(浙江农林大学亚热带森林培育国家重点实验室培育基地,临安 311300)
摘要:
为探究薄壳山核桃“小脚”现象的导管分子特性,采用组织离析法与显微照相技术,观察以‘Mahan’品种为砧木,‘Pawnee’品种为接穗的嫁接苗与非嫁接苗(‘Mahan’)导管分子类型及大小。结果表明,与非嫁接苗相比,嫁接苗砧木根段不同尾类型导管分子数目未见变化,两端壁水平导管分子数目多38.46%;砧木茎段两端无尾导管分子数目多50.00%,砧木茎段两端壁水平导管分子数目多92.31%;砧木根段平均倾斜角度比根小36.57%、砧木茎段平均倾斜角度比茎下部小30.56%;砧木根段平均长度比根短12.62%,平均宽度比根小4.18%,砧木茎段平均长度比茎下部短3.89%,平均宽度比茎下部宽11.68%。综上所述,嫁接薄壳山核桃“小脚”现象可能与砧木根段和砧木茎段木质部导管分子的形态与大小变化有关。
关键词:  薄壳山核桃  导管分子  “小脚”现象
DOI:10.13610/j.cnki.1672-352x.20161205.002
投稿时间:2015-11-02
基金项目:浙江省林业厅项目(2014B01)和浙江省农业新品种选育重大科技专项(2012C12904)资助。
Male flower development and pollen germination of ‘Mahan’ pecan
SUN Zhichao,CHENG Hui,CHANG Ailing,MI Yaoqi,HUANG Ruimin,WANG Zhengjia
(State Key Laboratory Breeding Base of Subtropical Forest Culture, Zhejiang Agriculture and Forestry University, Linan 311300)
Abstract:
In order to study the development of male flowers and pollen germination of pecan, ‘Mahan’ was systematically observed under microscope using paraffin sections for its staminal flowering habit. The structure and germination characteristics of the pollen grain were also observed using scanning electron microscope. The results showed that the male flower primordium took shape on the top of stamen in late March and began to differentiate in early April. In late April, the pollen sac formed in anther and the pollen mother cell began meiosis to tetrad stage. Then the tetrad produced pollen grains of single cell nucleus and the tapetum disappeared gradually in the developmental process. The pollen grain was fully developed when it had two nuclei. A stigma in the receptive period after pollination showed wave crests and papillary cells at the top of the carpel. Pollen germinated on a stigma four hours after pollination. More pollen germinated on stigma and pollen tubes grown toward ovary 12 hours after pollination. Pollen began collapse 24 hours after pollination and pollen tubes began collapse, and papillary cells at the top of the carpel began depression and collapse 3 days after pollination.
Key words:  Carya illinoinensis  vessel element  stock under growth

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