引用本文:[点击复制]
[点击复制]
【打印本页】 【在线阅读全文】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 69次   下载 176 本文二维码信息
码上扫一扫!
利用曲霉sp.HS-6发酵玉米秸秆进行固体纤维素酶的制备
薛银婷,何兴兵,林永慧,黄诗,罗永路
0
(吉首大学生物资源与环境科学学院,吉首 416000)
摘要:
以玉米秸秆为原料,分别以氯化铵、硫酸铵、硝酸铵、硝酸钾和尿素为氮源,设置了氮源的3个浓度梯度:0.5、1和2 g·L-1,对曲霉sp. HS-6产纤维素酶条件进行探究,主要确定了最佳发酵时间、最佳氮源种类和最佳氮源浓度,再利用得到的最佳条件,进行盐析,低温冷冻干燥等技术发酵得到酶制剂。结果表明,通过测定酶活,确定了该曲霉的最佳发酵时间为3 d,筛选得到最佳的氮源种类为硝酸铵,硝酸铵最佳的氮源浓度为0.5 g·L-1。基于此最佳条件,将60 g玉米秸秆粉末发酵培养3 d,最终从发酵液中提取得到纤维素酶干品酶制剂8.0 g,并计算出酶的回收率为48.0%。此实验对以后纤维素酶的规模化制备提供了一定的工艺参考。
关键词:  玉米秸秆  曲霉  纤维素酶  氮源  发酵
DOI:10.13610/j.cnki.1672-352x.20160512.023
投稿时间:2015-12-12
基金项目:国家自然科学基金(31360135, 31560205, 41501335), 湖南省高校重点实验室平台开放基金(12K107), 2013年湖南省大学生研究性学习和创新性实验计划项目(237)和湖南省战略性新兴产业科技攻关项目(2014GK1062)共同资助。
Production of solid cellulose through fermentation of maize straw by Aspergillus sp. HS-6
XUE Yinting,HE Xingbing,LIN Yonghui,,HUANG Shi
(College of Biology and Environmental Sciences, Jishou University, Jishou 416000)
Abstract:
In this paper, we explored the production conditions of cellulase through fermentation of maize straw by Aspergillus sp.HS-6 with the addition of NH4Cl, (NH4)2SO4, NH4NO3, KNO3, and CO(NH2)2 as nitrogen sources at three concentrations (0.5, 1 and 2 g·L-1). The optimal fermentation time, the optimal nitrogen source and Aspergillus sp.HS-6 concentration were determined. Based on the optimum fermentation conditions, salting-out and freeze-drying techniques were used to produce solid enzymes. The results showed that: the optimal fermentation time was 3 days by determining the enzyme activity and the optimal nitrogen source was NH4NO3 at 0.5 g·L-1. Under this condition, we obtained the product of solid cellulase with a recovery rate of 48.0%. This experiment would provide some references for a large-scale production of cellulase in the future.
Key words:  Aspergillus  cellulose  nitrogen source  maize straw  fermentation

用微信扫一扫

用微信扫一扫