引用本文:[点击复制]
[点击复制]
【打印本页】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览次   下载 本文二维码信息
码上扫一扫!
钌配合物[Ru(MeIm)4(4mopip)]2+稳定G-四链体 对抑制肺癌细胞生长机制的研究
孙冬冬,牟志鹏,李暖,张伟伟,赵志伟,黄东辉,杨小方,汪维云
0
(安徽农业大学生命科学学院,合肥 230036)
摘要:
制备一种新型钌配合物[Ru(MeIm)4(4mopip)]2+(RuMeMo),运用元素分析(C、H、N)、电喷雾电离质谱(ESI-MS)、核磁共振谱(1H NMR)对RuMeMo进行了结构表征。紫外光谱、热变性试验和圆二色光谱研究RuMeMo与F21T的分子作用机制,发现其能诱导线性G-四链体DNA形成混合型结构并能有效稳定G-四链体。MTT法评估RuMeMo对癌细胞的抑制作用及其对正常细胞的毒性,发现其对肺癌细胞(A549)表现出高选择的抑制作用,而对正常人类肺原胚细胞(CDDP)则表现出较低的细胞毒性。本研究结果表明,RuMeMo因其稳定G-四链体DNA的能力,从而有被运用于肺癌治疗的潜质。
关键词:  钌配合物  G-四链体  细胞凋亡  肺癌  机制
DOI:10.13610/j.cnki.1672-352x.20151224.020
投稿时间:2015-10-20
基金项目:国家自然科学基金青年科学基金(21401002), 安徽省自然科学基金青年项目(1508085QB37), 安徽农业大学青年科学基金重点项目(2013zr011)和安徽农业大学生物制药新专业建设(SJJD201313)共同资助。
Anti-tumor activity and mechanism of apoptosis of tumor cells induced by ruthenium complexes
SUN Dongdong,MOU Zhipeng,LI Nuan,ZHANG Weiwei,ZHAO Zhiwei,HUNAG Donghui,YANG Xiaofang,WANG Weiyun
(School of Life Science, Anhui Agricultrual University, Hefei 230036)
Abstract:
In this study, a new ruthenium complex, [Ru(MeIm)4(4mopip)]2+(RuMeMo), was designed, synthesized, and further characterized by Elemental analyses (C, H and N),ESI-MS and NMR. UV-Visible spectroscopy (UV-Vis), fluorescent resonance energy transfer (FRET), and circular dichroism (CD) were used to research the molecular mechanism of actions between RuMeMo and F21T. RuMeMo showed a high inhibitory activity to A549 and a low cytotoxicity activity to CDDP via MTT analysis. All results indicated that RuMeMo may be used for the treatment of lung cancer due to its ability for stabling G-complex.
Key words:  ruthenium complexes  G-quadruplex  apoptosis  lung cancer  mechanism

用微信扫一扫

用微信扫一扫