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辣椒疫霉可溶性蛋白和酯酶同工酶电泳分析
李萍,叶涛,郑婷,高智谋
0
(安徽农业大学植物保护学院,合肥 230036; 安庆职业技术学院园林园艺系,安庆 246003)
摘要:
采用SDS-聚丙烯酰胺凝胶电泳技术(SDS-PAGE)和PAGE电泳技术分别对安徽不同地区的46个辣椒疫霉进行可溶性蛋白和酯酶同工酶分析,从蛋白质和酶学水平上分析辣椒疫霉的生理生化特征。可溶性蛋白电泳表明,每个辣椒疫霉分离出了25~37条谱带,平均31.5条。其中具有多态性的条带共有24条,多态性比率达64.9%,可溶性蛋白图谱与地理来源相关,但与致病力不相关。酯酶同工酶PAGE电泳显示辣椒疫霉菌株各有1~8条条带,Rf值为0.05~1.00,不同辣椒疫霉菌株间某些同工酶谱带数和同一迁移率谱带的颜色和宽度差异显著,其中弱致病力菌株的条带数为1~3条,说明菌株的致病力差异可在酯酶同工酶水平上得到反映。
关键词:  辣椒疫霉  可溶性蛋白  酯酶同工酶  电泳图谱
DOI:10.13610/j.cnki.1672-352x.20151102.017
基金项目:安徽省自然科学基金项目(070411028)和农业部公益性行业科研专项(201303018)共同资助。
Analysis of the soluble protein and esterase isozyme of Phytophthora capsici Leonian
LI Ping,YE Tao,ZHENG Ting,GAO Zhimou
(School of Plant Protection, Anhui Agricultural University, Hefei 230036; Department of Horticulture and Landscape, Anqing Vocational and Technical College, Anqing 246003)
Abstract:
In order to analyse the physiological and biochemical differentiation among isolates of Phytophthora capsici at protein or esterase isozyme level, 46 isolates of the pathogenic oomycete collected from different localities of Anhui province were clustered based on their soluble protein and esterase isozyme profiles obtained through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and polyacrylamide gel electrophoresis (PAGE), respectively. The soluble protein analysis showed 25 to 37 main bands were obtained with each isolate, with average value of 31.5. Twenty-four of those 37 bands were polymorphic (64.9%), revealing high polymorphism among the isolates. The soluble protein group had a certain correlation with the localities of isolates, but no relation with the pathogenicity. The esterase isozyme analysis showed each isolate had 1 to 8 main bands and the Rf value ranged from 0.05 to 1.00. The obvious difference in the numbers of bands and activities of bands with the same Rf value was also detected. The bands of weakly pathogenic isolates were 1 to 3, which supported that esterase isozyme patterns could be indicators to reveal the difference of pathogen pathogenicity.
Key words:  Phytophthora capsici  soluble protein  esterase isozyme  electrophoresis pattern

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