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玉米抗病相关基因NPR1的同源克隆及表达分析
武健东,洪玲,江海洋,朱苏文
0
(安徽农业大学生命科学学院,安徽省作物生物学重点实验室,合肥 230036)
摘要:
NPR1是植物系统获得性(systemic acquired resistance, SAR)抗病反应中的关键基因, 对植物的广谱抗性起重要调控作用。以玉米自交系“齐319”为材料,通过PCR 方法克隆到一个玉米NPR1基因(命名为ZmNPR1)。序列分析结果显示ZmNPR1包含两个保守结构域POZ/BTB位点和Ankyrin repeat锚蛋白重复位点。蛋白质聚类分析表明ZmNPR1与水稻OsNPR2的同源性最高。亚细胞定位结果显示ZmNPR1定位于洋葱表皮细胞的细胞核中。半定量PCR结果显示,ZmNPR1和玉米防卫基因PAL(编码苯丙氨酸解氨酶)响应水杨酸、玉米矮花叶病毒和玉米小斑病原菌的诱导并显著上调表达;而ZmNPR1PAL的表达水平受到茉莉酸甲酯的明显抑制。这表明ZmNPR1在玉米中参与到水杨酸介导的抗病反应通路。
关键词:  玉米  NPR1  水杨酸  抗病性
DOI:10.13610/j.cnki.1672-352x.20150825.024
投稿时间:2015-06-10
基金项目:安徽省自然科学基金(1408085MKL35)和安徽省博士后研究人员科研项目共同资助。
Cloning and expression analysis of the disease-related gene NPR1 in maize
WU Jiandong,Hong Ling,JIANG Haiyang,ZHU Suwen
(Kay Laboratory of Crop Biology of Anhui Province, School of Life Science, Anhui Agricultural University, Hefei 230036)
Abstract:
The NPR1 gene plays a key role in systemic acquired resistance (SAR) and provides broad- spectrum resistance in plants. In this study, the NPR1 gene was cloned from the maize inbred line “Qi 319” based on PCR technique and was named as ZmNPR1. Sequence analysis exhibited two conserved POZ/BTB domains and an ankyrin repeat domain in the ZmNPR1 protein. Phylogenetic analysis showed that ZmNPR1 was high homology with rice OsNPR2. Transient expression of the ZmNPR1-GFP fusion protein in onion skin cells showed a nuclear localization of ZmNPR1. RT-qPCR showed that expression of ZmNPR1 and the gene PAL, which encodes a pathogenesis-related protein, were induced by the treatment of salicylic acid (SA), Maize mosaic virus and Maize Helminthosporium maydis, whereas the expression level of ZmNPR1 was inhibited by methyl jasmonate. Taken together, these results suggest that the ZmNPR1 gene plays an important role in the SA-meditated disease resistance pathway in maize.
Key words:  maize  NPR1  SA  disease resistance

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