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喷钛对甜叶菊糖苷含量和积累量的影响
马婷婷,张咪咪,李鹏,魏亚,常江
0
(安徽农业大学资源与环境学院,合肥 230036)
摘要:
为探索喷钛对甜叶菊叶片中糖苷、甜菊苷(St)和莱鲍迪苷 A(RA)的含量、积累量及其比例的影响,为中药材的施肥提供科学依据,应用盆钵培养方法,在甜叶菊生长前期和中期进行叶面喷钛。结果表明:菊隆5号的最佳喷施区间12.22 ~19.36 mg·kg-1,在喷钛浓度为19.36 mg·kg-1时含量达到最高;新品种2号的最佳喷施区间为12.10 ~16.71 mg·kg-1,在喷钛浓度为16.71 mg·kg-1时含量达到最高;喷钛后新品种2号St所占比例的最高增幅为156.71%,菊隆5号St的最高增幅为42.28%。喷钛可明显提高糖苷含量和积累总量,与对照相比,差异显著;经回归发现糖苷含量或积累量与喷钛浓度间存在二次曲线关系,相关极显著;喷钛可以增加2个品种糖苷中St的比例。
关键词:  螯合钛  莱鲍迪苷A  甜菊苷  高效液相色谱  经济效益
DOI:10.13610/j.cnki.1672-352x.20150625.015
投稿时间:2015-03-13
基金项目:安徽省中草药产业体系项目资助。
Effect of spraying titanium on the glycoside?content and accumulation in stevia?leaves
MA Tingting,ZHANG Mimi,LI Peng,WEI Ya,CHANG Jiang
(School of Resources and Environmental Science, Anhui Agricultural University, Hefei 230036)
Abstract:
In order to provide a scientific basis of fertilization for traditional Chinese medicinal plants, an indoor?simulation experiment?was carried out. A titanium solution was sprayed on the leaf surface of stevia at the early and middle growth?stages. The effects of different concentrations of titanium on the contents, accumulation, and proportion of glycoside, St, and RA in the stevia leaf were studied. Results showed that the optimum concentration of spraying titanium was in the range of 12.22 -19.36 mg·kg-1 for Julong No.5 and 12.10 -16.71 mg·kg-1 for variety?No.2, respectively and the maximum?content of glycoside was obtained when the concentration reached to 19.36 mg·kg-1 and 16.71 mg·kg-1, correspondingly. The?highest?increase rate of St after titanium spraying was 156.71% in new variety?No.2 and 42.28% in Julong No.5. In conclusion, the?glycoside?content and accumulation in the stevia?leaf were significantly?increased by spraying titanium. There?was a?significant?statistical?difference between the treatment and the control was observed. Regression?analysis indicated that a?significant?correlation and quadratic?curve?relation between glycoside content and accumulation and titanium concentration existed. The proportion of St in glycosides in both varieties was increased by titanium application.
Key words:  titanium chelate  rcbaudioside A  stevioside  high performance liquid chromatography (HPLC)  economic benefits

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