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红曲金钗石斛双向发酵产品抗氧化活性的研究
侯素媛,周礼红,刘磊
0
(贵州大学药学院,贵阳 550025;贵州大学生命科学院,贵阳 550025)
摘要:
为筛选适合与金钗石斛双向发酵的红曲霉菌株,研究双向发酵的药性菌质是否具有高抗氧化活性,采用ABTS法、DPPH法和抗脂质过氧化法3种体外抗氧化模型比较2株具有抗氧化活性的红曲霉菌株与金钗石斛双向发酵药性菌质抗氧化活性。结果表明,Vc对照组的ABTS清除率和DPPH清除率明显高于各发酵组及金钗石斛组,而抗脂质过氧化抑制率却低于2株不同红曲霉菌株与金钗石斛双向发酵药性菌质,具有极显著性差异(P<0.01);2株不同红曲霉菌株与金钗石斛双向发酵药性菌质的3种体外抗氧化活性均高于红曲霉单独发酵产品和金钗石斛,尤其菌株GZUM-123双向发酵药性菌质的抗脂质过氧化抑制率提高最多为30.09%,具有极显著性差异(P<0.01)。此研究可为新型双向发酵的抗氧化产品的开发提供一定的依据。
关键词:  红曲霉  金钗石斛  抗氧化活性
DOI:10.13610/j.cnki.1672-352x.20150625.011
基金项目:上海市科委科技支撑项目“新型微生物农药的研发与开发”(13391901700)资助。
Antioxidant activity of?mycoplasm from bidirectional fermentation of Monascus and Dendrobium nobile Lindl.
HOU Suyuan,ZHOU Lihong,LIU Lei
(College of Pharmaceutical Sciences, Guizhou University, Guiyang 550025;College of Life Sciences, Guizhou University, Guiyang 550025)
Abstract:
In order to screen strains from a bidirectional fermentation of Monascus and Dendrobium nobile and study the antioxidant activity of mycoplasm from a bidirectional fermentation medicine, three antioxidative modes in vitro, ABTS free radical scavenging, DPPH free radical scavenging, and inhibiting lipid peroxidation, were compared. Results showed that Vc group had higher ABTS and DPPH scavenging ratios compared to bidiretional ferment products, Monascus ferment products and Dendrobium nobile, while its anti-lipoperoxidation inhibition ratio was lower than those from the bidiretional fermentation medicinal mycoplasm of the two strains(P<0.01. The bidirectional fermentation medicinal mycoplasm from two?Monascus strains and Dendrobium nobile had a higher antioxidant activity compared to individual Monascus ferment products and Dendrobium nobile. In three modes in vitro, the two-way fermentation medicinal mycoplasm of strain GZUM-123 had the highest inhibiting lipid peroxidation with an increase of 30.09% (P<0.01). The study can provide a foundation for the development of a new bidirectional fermentation.
Key words:  Monascus  Dendrobium nobile Lindl  antioxidant activity

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