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液体发酵因子对棘孢木霉Tr148c分生孢子产量的影响
王军,旷文丰,陈晨,王承芳,毛伟力
0
(上海万力华生物科技有限公司,上海 201203; 安徽农业大学,合肥 230036)
摘要:
研究营养成分(碳源和氮源)、初始pH、温度、转速、装液量和接种量等因子对棘孢木霉菌株Tr148c在液体发酵过程中分生孢子产量的影响。首先采用马铃薯液体培养基(PDB)对以上各单因子进行测试,并在此实验基础上进一步采用3水平4因子的正交试验,对碳源、氮源、装液量和转数等因子进行优化测试。结果表明,综合因子优化配方和发酵条件为: 当培养时间144 h时,甘露醇( 30 g·L-1)、酵母粉( 1 g·L-1)、初始pH为6、温度30℃、转速200 r·min-1、装液量150 mL/500 mL、接种量4%(v/v),产分生孢子量可达到2×108个·mL-1
关键词:  棘孢木霉菌  Tr148c  产分生孢子量  液体发酵
DOI:10.13610/j.cnki.1672-352x.20150626.007
基金项目:上海市科委科技支撑项目“新型微生物农药的研发与开发”(13391901700)资助。
Effect of liquid fermentation factors on conidial production of Trichoderma asperellum strain Tr148c
WANG Jun,KUANG Wenfeng,CHEN Chen,WANG Chengfang,MAO Weili
(Shanghai W.H.L.Bio-tech Corp., Shanghai 201203; Anhui Agricultural University, Hefei 230036)
Abstract:
A strain of Trichoderma asperellum-Tr148c isolated from a soil sample by Wanlihua Bio-Tech Company was used to study the effect of the factors of nutrient sources (carbon and nitrogen), initial pH, temperature, rotating speed and volumes of loaded liquid and inoculum on the production of conidial spores of Tr148c during the fermentation process. Potato dextrose broth (PDB) was initially used as a basic culture medium to test each individual factor, and an orthogonal test with four factors at three levels was then utilized. The results showed that the highest production of conidial spores (2.0×108 cfu·mL-1) was obtained after 144 h liquid cultivation with an optimal combination of the factors within a formula during the fermentation process, which are the nitrogen and carbon sources of mannitol 30 g·L-1 and yeast powder 1 g·L-1, respectively; initial pH, 6; temperature, 30℃; rotatingspeed: 200 r·min-1; volumes of loaded liquid and inoculums: 150 mL/500 mL and 4% (v/v), respectively.
Key words:  Trichoderma asperellum  Tr148c  conidial production  liquid fermentation

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