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土壤自然干旱及复水对通关藤抗旱能力的影响
孟衡玲,杨生超,张薇,龙光强,孟珍贵
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(红河学院,云南省高校农作物优质高效栽培与安全控制重点实验室,红河 661100;云南农业大学,云南省优势中药材规范化种植工程研究中心,昆明 650201)
摘要:
以通关藤为材料,设计4个实验处理组,分析通关藤在干旱胁迫及复水后抗旱生理指标的变化,为通关藤的仿野生栽培提供理论指导。结果表明,脯氨酸及可溶性糖含量随着胁迫程度的加剧,呈“先升高后降低”的变化趋势,2个指标均在第8天时达到最大值,各胁迫处理的含量均显著高于对照;MDA含量呈“先升高后降低再升高”的趋势,胁迫第8~12 天时MDA含量显著低于对照;SOD和POD在整个胁迫期内活性均显著高于对照,复水后其活性有所降低,但仍高于对照。上述变化结果表明,干旱胁迫8~12 d达到通关藤抗旱高峰,随后抗旱能力下降,胁迫解除后自身修复能力较强。
关键词:  通关藤  自然干旱  复水  抗旱能力
DOI:10.13610/j.cnki.1672-352x.20150626.002
基金项目:云南圣和植物药业有限公司项目(20120523)和云南省科技厅应用基础研究项目(2012FB174)共同资助。
Effects of drought stress and rewatering on drought tolerance of Marsdenia tenacissima
MENG Hengling,YANG Shengchao,ZHANG Wei,LONG Guangqiang,MENG Zhengui
(The Key Laboratory of High Quality Crops Cultivation and Safety Control, Honghe University, Honghe 661100;Yunnan Provincial Good Agricultural Practice Engineering Research Center of Dominant Chinese Medical Materials, Yunnan Agricultural University, Kunming 650201)
Abstract:
In order to provide a theoretical direction for cultivation of M. tenacissima in an imitating wild condition, four experimental treatment groups were designed to analyze physiological changes under different degrees of drought stress and rewatering. The results indicated that proline and soluble sugar contents quickly increased to the peak 8 days after the drought treatment and then decreased along with an increase of the drought stress. The contents of proline and soluble sugar were significantly higher than that of the control during the stress period. The MDA content was significantly lower than that of the control from the 8th to 12th days after treatment. SOD and POD enzymes showed a higher activity during the drought stress period compared to the control. The MDA content showed a pattern of increase-decrease-increase. All physiological stresses were relieved after rewatering. It is?deduced that M. tenacissima could tolerate drought and has a strong ability to recover from drought stress.
Key words:  Marsdenia tenacissima  natural drought  rewatering  drought tolerance

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