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草莓茎尖培养快繁体系的研究
翟婷婷,刘成连,原永兵,屈海泳,沈俊岭,刘强
0
(青岛农业大学园艺学院,青岛 266109)
摘要:
以‘红颜’和‘甜查理’的匍匐茎茎尖为试材,诱导出丛生芽后进行增殖培养和生根培养,研究不同激素浓度及组合对茎尖增殖和生根的影响。结果表明,最适宜红颜增殖的培养基为MS+0.8 mg·L-1 6-BA +0.1 mg·L-1 NAA;最适宜甜查理增殖的培养基为MS+0.3 mg·L-1 6-BA+0.5 mg·L-1 NAA。最适宜红颜草莓生根的培养基是1/2 MS+ 0.8 mg·L-1 IBA;最适宜甜查理草莓生根的培养基是1/2 MS+ 0.1 mg·L-1 IBA。
关键词:  草莓  茎尖培养  快繁
DOI:10.13610/j.cnki.1672-352x.20150626.014
投稿时间:2015-04-23
基金项目:山东省农业良种工程项目资助。
Rapid propagation system of meristem culture for strawberry
ZHAI Tingting,LIU Chenglian,YUAN Yongbing,QU Haiyong,SHEN Junling,LIU Qiang
(School of Horticulture, Qingdao Agricultural University, Qingdao 266109)
Abstract:
In this paper, the effects of different hormones and the concentrations on the proliferation and rooting of strawberry were studied using the stolons tips of ‘Benihoppe’and ‘Sweet Charlie’ as materials. The result showed that the medium composed of MS+0.8 mg·L-1 6-BA +0.1 mg·L-1 NAA was best for ‘Benihoppe’, and MS+0.3 mg·L-1 6-BA+0.5 mg·L-1 NAA was best for ‘Sweet Charlie’ proliferation. 1/2 MS+0.8 mg·L-1 IBA was the best for rooting of ‘Benihoppe’, and 1/2 MS+0.1 mg·L-1 IBA was best for that of ‘Sweet Charlie’.
Key words:  strawberry  meristem culture  rapid propagation

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