引用本文:[点击复制]
[点击复制]
【打印本页】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览次   下载 本文二维码信息
码上扫一扫!
16种山羊和6种绵羊GDF9基因G7和FecGV突变检测
金慧慧,储明星,潘章源,狄冉,刘秋月,胡文萍,王翔宇,赵永聚
0
(西南大学动物科技学院,重庆 400715;中国农业科学院北京畜牧兽医研究所,农业部畜禽遗传资源与种质创新重点实验室,北京 100193)
摘要:
生长分化因子9(growth differentiation factor 9,GDF9)是第一个被发现的由卵母细胞分泌的生长因子,能直接影响动物的产仔数。G7或c.1111G>A突变是Belclare和Cambridge绵羊GDF9基因编码区1111 bp处发生的G→A突变,导致第371位氨基酸由Val改变为Met(V371M)。G7突变对挪威白绵羊的产羔数有显著影响。FecGV是高产的Ile de France绵羊GDF9基因编码区943 bp处发生的C→T突变(c.943C>T),导致非保守氨基酸Arg改变为Cys(p.Arg315Cys)。FecGV突变杂合子绵羊的排卵数和产羔数显著高于野生型。采用测序方法检测FecGV突变和PCR-RFLP方法检测G7突变在16个山羊品种(济宁青山羊、贵州白山羊、成都麻羊、萨能奶山羊、板角山羊、关中奶山羊、辽宁绒山羊、大足黑山羊、古蔺马羊、波尔山羊、内蒙古绒山羊、金堂黑山羊、川东白山羊、安哥拉山羊、圭山山羊和太行山羊)和6个绵羊品种(小尾寒羊、洼地、湖羊、杜泊、黑萨福克和中国美利奴)中的分布情况。结果表明,在16个山羊品种和6个绵羊品种中均未检测到G7和FecGv突变,提示GDF9基因G7和FecGv突变对以上山羊和绵羊品种的繁殖力均没有显著影响。
关键词:  山羊  绵羊  GDF9基因  G7突变  FecGV突变  PCR-RFLP
DOI:10.13610/j.cnki.1672-352x.20141225.005
基金项目:中国农业科学院科技创新工程(ASTIP-IAS13)和国家肉羊产业技术体系专项资金(CARS-39)共同资助。
Detection of the G7 and FecGV mutations of the GDF9 gene in sixteen goat and six sheep breeds
JIN Huihui,CHU Mingxing,PAN Zhangyuan,DI Ran,LIU Qiuyue,HU Wenping,WANG Xiangyu,ZHAO Yongju
(College of Animal Science and Technology, Southwest University, Chongqing 400715;Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193)
Abstract:
The GDF9 is the first discovered growth factor which was secreted by oocytes and could affect the litter size (LS) of animals. Sequence analyses of the GDF9 coding region showed a single nucleotide polymorphism (G7 or c. 1111 G > A) in Belclare and Cambridge sheep, responsible for a Val → Met substitution at position 371 (V371M). The G7 mutation in GDF9 was strongly associated with LS in Norwegian White Sheep. FecGV (c.943C>T) was identified in prolific Ile de France ewes, resulting in a non-conservative amino acid change (p.Arg315Cys) in the cleavage site of the polypeptide. FecGV heterozygotes ewes had a higher rate of ovulation (OR) and LS than wild-type ewes. The aim of this study was to detect the distribution of the FecGV mutation by re-sequencing. The G7 mutation of the GDF9 gene in sixteen goat breeds (Jining Grey, Guizhou White, Dazu Black, Gulin Ma, Matou, Boer, Chengdu Ma, Saanen Dairy, Inner Mongolia Cashmere, Banjiao, Jintang Black, Guanzhong Dairy, Chuandong White, Angora, Liaoning Cashmere, Guishan and Taihang goats) and six sheep breeds (Small Tail Han, Wadi, Hu, Dorper, Black Suffolk and Chinese Merino sheep) was also detected using the PCR-RFLP method. Results showed that both G7 and FecGV mutations of GDF9 were not detected in all sixteen goat and six sheep breeds tested, indicating that the G7 and FecGV mutations had no significant effect on fecundity of these sixteen goat and six sheep breeds.
Key words:  goat  sheep  GDF9 gene  G7 mutation  FecGV mutation  PCR-RFLP

用微信扫一扫

用微信扫一扫