引用本文:[点击复制]
[点击复制]
【打印本页】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览次   下载 本文二维码信息
码上扫一扫!
RNA病毒诱导的烟草WRKY转录因子基因的应急表达
周汉琛,席玉珍,魏书
0
(安徽农业大学茶与食品科技学院,合肥 230036)
摘要:
植物和病毒在基因水平上的互作是植物感病和抗病的分子基础。以模式植物烟草及其3种RNA病毒(马铃薯Y型病毒,Potato virus Y,PVY;烟草花叶病毒,Tobacco mosaic virus,TMV;黄瓜花叶病,Cucumber mosaic virus,CMV)为试材,旨在研究受病毒共同诱导、并可能在植物抗病反应中具有重要功能的转录因子基因。试验首先获得10个受病毒诱导的烟草同源基因。在比较了3种病毒侵染、低温和盐害协迫以及逆境信号物质(茉莉酸甲酯与水杨酸)处理对上述基因表达的影响后发现, 3种RNA病毒所诱导的植物基因有所不同。但属于WRKY基因家族的06G基因受3种病毒的共同、高效诱导,其表达水平与叶片发育程度呈负相关。研究结果表明, 该基因在植物病毒病发生及植物抗病分子育种中可能有重要意义。
关键词:  RNA病毒  防御反应  烟草  WRKY转录因子
DOI:10.13610/j.cnki.1672-352x.20141029.033
投稿时间:2014-03-27
基金项目:国家自然科学基金(31070614)和教育部博士点项目(20123418110002)共同资助。
Expression of a novel WRKY gene in response to three RNA viruses in tobacco
ZHOU Hanchen,XI Yuzhen,WEI Shu
(School of Tea and Food Technology, Anhui Agricultural University, Hefei 230036)
Abstract:
Gene between viruses and plants is the molecular basis of plant susceptibility and resistance to virus diseases. In this study, three RNA viruses (potato virus Y-PVY, tobacco mosaic virus-TMV, and cucumber mosaic virus-CMV) were used to study virus-induced transcription factor genes in Nicotiana tabacum. These genes may play an important role in plant defensive responses to virus infection. Based on public database and literature, ten tobacco genes homologous to the virus-induced genes previously identified in N. benthamiana and Oryza sativa were found. Quantitative analyses of the gene transcripts responding to virus infection, abiotic stresses, and stress related compounds (1 mmol·L-1 of methyl jasmonic acid or salicylic acid solution) revealed that each virus had its specific expression profile of induced plant genes. Tobacco 06G gene, a member of WRKY subfamily II, was quickly and effectively induced by all three viruses. Expression of the 06G gene was higher in young leaves than in old ones. Our data suggest that this novel WRKY gene may function as a regulator in plant-virus interactions and such interactions can be utilized to enhance virus resistance in plants.
Key words:  plant virus  resistance response  tobacco  WRKY transcription factor

用微信扫一扫

用微信扫一扫