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人工培养长座线虫草子实体及其菌丝体的挥发性成分分析
张颖裕,刘洋,李扬眉,张龙娃,李春如
0
(安徽农业大学微生物防治省重点实验室,合肥 230036)
摘要:
首次采用同时蒸馏萃取法(SDE)提取了人工培育的长座线虫草子实体及摇瓶菌丝体的挥发性成分,并用气相色谱质谱联用仪(GC-MS)进行了分析。结果表明,不同培养方式对长座线虫草挥发性物质的数目和含量有较大影响,对挥发性成分的类型影响较大,液体摇瓶菌丝体挥发性物质种类主要有:酮类、醇类、烷烃类、烯烃类、醌类和醚类化合物;子实体中挥发性物质种类主要有:醇类、醛类和酚类。液体摇瓶菌丝体鉴定出20种挥发性物质,以1-辛烯-3-醇(37.70%)为主,人工培养子实体鉴定出6种挥发性物质,以1-辛烯-3-醇(51.93%)和2,6-二叔丁基对甲苯酚(38.37%)为主。2种人工培养物挥发性物质的共有成分为共有成分1-辛烯-3-醇、2,6-二叔丁基对甲苯酚在菌丝体中占51.66%,在子实体的挥发性成分中占90.30%。
关键词:  长座线虫草  菌丝体  子实体  挥发性成分  SDE  GC-MS
DOI:10.13610/j.cnki.1672-352x.20141029.027
投稿时间:2014-03-04
基金项目:国家自然科学基金(30570004), 国家高技术研究发展计划项目(863计划)(2007AA021506)共同资助。
Volatile components in mycelia and fruiting bodies of the artificial culture of Ophiocordyceps longissima
ZHANG Yingyu,LIU Yang,LI Yangmei,ZHANG Longwa,LI Chunru
(Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036)
Abstract:
The volatile compounds were extracted from the mycelia and fruiting bodies of the artificial culture of Ophiocordyceps longissima using simultaneous distillation and extraction (SDE) method. Their volatile components were analyzed using gas chromatography-mass spectrometry (GC-MS). The results showed that culture conditions had great influence on the content and number of volatile components; however, little impact on component classes was observed. The main volatile substances in mycelia were ketones, alcohols, alkenes, phenols and ethers, while alcohols, aldehydes, and phenols are main volatile compounds in fruiting bodies. Twenty compounds were detected in the mycelia liquid in which the content of 1-octene-3-alcohl (37.70%) was the highest. Six compounds were identified from the artificial culture of the fruiting bodies with the content of 1-octene-3-alcohl (51.93%) and butylated hydroxytoluene (38.37%) being relatively high. The compounds of 1-octen-3-ol and butylated hydroxytoluene are two common constituents in both artificial cultures and their contents were accounted for 51.66% and 90.30% of the total volatile components in mycelia and fruiting body, respectively.
Key words:  Ophiocordyceps longissima  fruiting body  mycelia  volatile component  SDE  GC-MS

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