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台湾线虫草人工培养物及其培养基质成分分析
李扬眉,丁海燕,张颖裕,李春如
0
(安徽农业大学微生物防治省重点实验室,合肥 230036)
摘要:
对台湾线虫草摇瓶培养菌丝体、深层发酵菌丝体、固体培养子实体及除去子实体后的培养基质等4种样品的多种成分进行了比较分析。结果表明,粗脂肪、多糖在摇瓶菌丝体与发酵菌丝体的含量相当,均高于子实体;粗蛋白在摇瓶菌丝体、深层发酵菌丝体和子实体中的含量接近且较丰富;甘露醇和胞苷在深层发酵菌丝体中的含量较高,分别为3.41和2.44 mg·g-1;麦角甾醇在子实体中含量最高,达6.75 mg·g-1;腺苷含量比较结果为,摇瓶菌丝体(1.57 mg·g-1)>子实体(1.32 mg·g-1)>深层发酵菌丝体(0.96 mg·g-1) >培养基质(0.61 mg·g-1);常量元素Ca、Mg在摇瓶菌丝体(3.30×102, 3.98×103 μg·g-1)、深层发酵菌丝体(6.71×102, 3.00×103 μg·g-1)和子实体(1.51×103, 3.2×103 μg·g-1)的含量较为丰富;微量元素Zn、Cu在子实体中的含量最高,Fe、Mn、Ni和Se在深层发酵菌丝体中的含量最高;摇瓶菌丝体中的As和Cd及深层发酵菌丝体中的As含量均低于0.20 μg·g-1;子实体中未检测出Pb的含量。
关键词:  台湾线虫草  黄山被毛孢  子实体  菌丝体  有效成分
DOI:10.13610/j.cnki.1672-352x.20141029.006
投稿时间:2014-03-09
基金项目:国家自然科学基金(30570004)和国家高技术研究发展计划项目(863计划)(2007AA021506)共同资助。
Effective components in artificial culture of Ophiocordyceps formosana and its culture media
LI Yangmei,DING Haiyan,ZHANG Yingyu,LI Chunru
(Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036)
Abstract:
Various components in four samples of Ophiocordyceps formosana artificial cultures, i.e., flask culture mycelia, fermentative mycelia, solid culture fruiting bodies, and the culture media, were determined. The result showed that the contents of crude fat and polysaccharides were similar in flask culture mycelia and fermentative mycelia, higher than those in fruiting bodies. The content of crude protein was high and similar in flask culture mycelia, fermentative mycelia, and fruiting bodies. The contents of mannitol (3.41mg·g-1) and cytidine (2.44 mg·g-1) were highest in fermentative mycelia and ergosterol (6.75 mg·g-1) was highest in fruiting bodies. The content of adenosine was 1.57 mg·g-1 in flask culture mycelia, 1.32 mg·g-1 in fruiting bodies, 0.96 mg·g-1 in fermentative mycelia, and 0.61 mg·g-1 in solid culture residues. The contents of macro-elements Ca and Mg were high in flask culture mycelia (3.30×102, 3.98×103 μg·g-1), fermentative mycelia (6.71×102, 3.00×103 μg·g-1), and fruiting bodies (1.51×103, 3.2×103 μg·g-1). The contents of micro-elements Zn and Cu were highest in fruiting bodies and Fe, Mn, Ni and Se were highest in fermentative mycelia. The contents of As and Cd in flask culture mycelia and As in fermentative mycelia were lower than 0.20 μg·g-1. The content of Pb in fruiting bodies was too low to be detected.
Key words:  Ophiocordyceps formosana  Hirsutella huangshanensis  fruiting bodies  mycelia  effective components

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