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外源细胞分裂素BA对小白菜硫代葡萄糖苷含量的影响
胡克玲,朱祝军
0
(安徽农业大学园艺学院,合肥 230036;浙江农林大学农业与食品科学学院,临安 311300)
摘要:
研究外源细胞分裂素BA对小白菜硫代葡萄糖苷(硫苷)含量的影响,为提高小白菜硫苷含量提供参考。以不同浓度(0,0.2,5和20 mg·L-1) BA进行处理,分析其对小白菜生长和硫苷含量的影响。随BA浓度的增加,小白菜鲜重有增加的趋势,其中5~20 mg·L-1 BA处理显著增加了小白菜地上部鲜重。与对照相比,较高BA处理浓度(20 mg·L-1)显著诱导了5-甲基亚磺酰戊基硫苷、3-丁烯基硫苷、4-戊烯基硫苷和总硫苷的含量,但在0.2 mg·L-1 BA处理显著增加了4-戊烯基硫苷和2-苯乙基硫苷含量。5~20 mg·L-1 BA处理显著抑制了1-甲氧基吲哚-3-甲基硫苷的含量,但对其他吲哚族硫苷含量并没有引起显著的变化。不同种类硫苷对不同浓度的BA处理有不同的响应,其中20 mg·L-1 BA处理显著提高了小白菜总硫苷含量。
关键词:  小白菜  硫苷  生长  细胞分裂素
DOI:10.13610/j.cnki.1672-352x.20141029.004
基金项目:国家自然科学基金(30871718), 浙江农林大学科研发展基金(2008FR028)和安徽省高等教育振兴计划项目(2013zdjy057)共同资助。
Effects of BA on glucosinolate contents in pakchoi (Brassica campestris L. ssp. chinensis var. communis)
HU Keling,ZHU Zhujun
(School of Horticulture, Anhui Agricultural University, Hefei 230036;School of Agricultural and Food Science, Zhejiang Agricultural and Forestry University, Lin’an, 311300)
Abstract:
Effects of BA at 0, 0.2, 5, and 20 mg·L-1 on growth and glucosinolate content of pakchoi (cv. Shanghaiqing) were studied. The weight of pakchoi shoots was increasing as the increasing concentration of exogenous BA. BA at 5-20 mg·L-1 significantly increased the weight of pakchoi shoots. Compared to the control, the contents of glucoalyssin, gluconapin, glucobrassicanapin, and total glucosinolate were significantly increased when treated with 20 mg·L-1 BA. BA at 0.2 mg·L-1 also increased the contents of glucobrassicanapin and aromatic glucosinolate. However, 5-20 mg·L-1 BA treatment significantly inhibited the synthesis of neoglucobrassicin, but no effect was observed on other indole glucosinolates. Different glucosinolates responded differently to different BA concentrations in which 20 mg·L-1 BA treatment significantly increased the total glucosinolate content in pakchoi.
Key words:  glucosinolate  pakchoi  growth  BA

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