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‘傲雪’金银木组培快繁技术研究
左利娟,石进朝,陈兰芬,马喆
0
(北京农业职业学院园艺系,北京 102442)
摘要:
以‘傲雪’金银木带腋芽的半木质化茎段为材料,研究外植体消毒方法、不同植物生长调节剂对其继代增殖与生根的影响。结果表明:(1)先用75%的乙醇浸泡30 s,无菌水冲洗4 ~ 6次,然后加入0.1% HgCl2杀菌7 min,褐化与污染率较低;(2)增殖最佳培养基为MS + 6-BA 1.0 mg·L-1 + NAA 0.15 mg·L-1 + GA3 0.5 mg·L-1,增殖系数可达2.93;(3)生根最佳培养基为1/2MS+ IBA1.5 mg·L-1,生根率达93.9%。以上培养基均添加蔗糖30 g·L-1,琼脂7 g·L-1,pH值为6.0。
关键词:  ‘傲雪’金银木  增殖  生根
DOI:10.13610/j.cnki.1672-352x.20140620.034
投稿时间:2013-12-02
基金项目:2013年北京市属高等学校人才强教深化计划——知名专家(教学名师)建设项目(PXM2013_157203_000005)资助。
Establishment of an in vitro propagation system for Lonicear macckii ‘Aoxue’
ZUO Lijuan,SHI Jinchao,CHEN Lanfen,MA Zhe
(Department of Horticulture, Beijing Vocational College of Agriculture, Beijing 102442)
Abstract:
In this paper, semi-lignified stems were used to initiate an in vitro culture of Lonicear macckii ‘Aoxue’. Effects of surface sterilization methods and plant growth regulators on induction of adventitious buds, shoot multiplication, and rooting were determined. The results indicated: (1) Low contamination and browning of stem explants were observed when they were surface-sterilized with 75% ethanol for 30 s followed by washing with sterile water for 4-6 times, and then soaking in 0.1% mercuric chloride (HgCl2) for 7 min; (2)The best medium for shoot proliferation was MS + 6-BA 1.0 mg·L-1+ NAA 0.15 mg·L-1+ GA3 0.5 mg·L-1, with a proliferation rate of 2.93; (3)The rooting rate of plantlets was up to 93.9% in ? strength of MS medium supplemented with 1.5 mg·L-1 IBA. All media used contained 30 g·L-1 sucrose and 7 g·L-1 agar. The medium pH was adjusted to 6.0 before autoclaving.
Key words:  Lonicear macckii ‘Aoxue’  proliferation  rooting

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