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沙棘果渣中异鼠李素超声辅助提取工艺研究
胡丹,何珺,杨佳年
0
(贵州省生化工程中心,贵阳 550025; 贵州大学生命科学学院,贵阳 550025)
摘要:
采用超声波法提取沙棘果渣中异鼠李素。首先采用单因素对超声频率、提取时间、次数、提取温度、提取料液比及提取溶剂进行筛选,再对筛选提取条件进行正交验证试验。确定了该方法提取沙棘果渣中异鼠李素的参数,即50℃,超声频率40 kHz的条件下用V乙酸乙酯:V95%乙醇 为4:6的提取液超声2次,每次1 h,料液比1:25,所得的异鼠李素含量可达5.09 mg·g-1
关键词:  沙棘果渣  异鼠李素  提取
DOI:10.13610/j.cnki.1672-352x.20140620.020
基金项目:贵州省科技计划项目[黔科合SY字[2013]3014]资助。
Extraction of isorhamnetin from marc of Hippophae rhamnoides L. using ultrasound-assisted method
HU Dan,HE Jun,YANG Jianian
(Research Center of Biochemistry Engineering of Guizhou Province, Guiyang 550025; College of Life Sciences of Guizhou University, Guizhou University, Guiyang 550025)
Abstract:
The ultrasound-assisted method was used to extract isorhamnetin from marc of the Hippophae rhamnoides fruits. A single factor experiment was conducted to select ideal ultrasonic frequency, extraction time and frequency, extraction temperature, solid-liquid ratio, and extraction solvent. These extraction conditions were then verified with an orthogonal experiment. The best conditions of the ultrasonic method for isorhamnetin extraction from Hippophae rhamnoides marc were: the ultrasonic frequency of 40 kHz, the extraction temperature of 50 ℃, V ethyl acetate: V95% ethyl alcohol of 4:6 as the extraction solvent, two extractions with one hour each, and the solid-liquid of 1:25. Under these conditions, the yield of isorhamnetin extraction can reach 5.09 mg·g-1.
Key words:  marc of Hippophae rhamnoides L.  isorhamnetin  extraction

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